FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGTAACAGGCAAACTATAAACAAGACACACAGTAAACACAAAAAACC13.225806451612903No Hit
GTACCGGCTTCTGGTTGCCTTAGGGACAAGAGCCTGAAATGGTTCAAATT13.225806451612903No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT13.225806451612903No Hit
AATATGGAGATTTTGGGTAGTATAGACCTCTTAACAATATTGTTCTTCCA13.225806451612903No Hit
GTCATAGTGTTGGCTCCATGTGCTGTAGGGCCCTGGGTTCTCAGGGTCTC13.225806451612903No Hit
CTAGTTTTCAGTGTATAGATTTTTTAATTCTTTGGTTATCTTTATTCCTA13.225806451612903No Hit
GAATTCACACAGAGCTTCAAGTTCTTCTTTGTCCCGATTTTGGGAGCAGA13.225806451612903No Hit
CCTATGAACACAGGTGTACAAATAACCTTTTGAAACCCTGCTTTCATTTC13.225806451612903No Hit
ATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATTC13.225806451612903No Hit
CAGGGCTTGATCTTATTACTCTGAGAGTATGACCTTATATGAAATTAATA13.225806451612903No Hit
GGGGGATTGCGTGAGGTTGGGTGCCCAGCCGGCCCAGCACCGCATCGCGC13.225806451612903No Hit
TGGTTCTGCCTCCCCCTCCCGCTTCACACCCCCCGTCGGTTGTGGCTTTG13.225806451612903No Hit
AACACACTCTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCA13.225806451612903No Hit
CATCTACATATGATCTAAACTGTGAACTGATGTGTAATGTAGGTTCGAGA13.225806451612903No Hit
GGCTCACTGCACCCCCAGCAAAGACCTCAGCCTGGAGCGGCCCTCCTTTC13.225806451612903No Hit
TACTAATACATTAAACAAACATCCAAAATACAAAATATTTCAGCAAACTC13.225806451612903No Hit
GTATTGGGGGTAGGAGCTACAGTAGCGAGGGCAGAAAGCATTGCTTCCTT13.225806451612903No Hit
CAGAATGAAGTGGATGATGGCAATGAGATCCGCAATTACAGCAGACTAGA13.225806451612903No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT13.225806451612903No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT13.225806451612903No Hit
TACAAACCCTGGCCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTGA13.225806451612903No Hit
CGTATACATAGAGAAAAGTAAAACTAACCCTTGTTTCACCATAGGTGACC13.225806451612903No Hit
GAAGCAATTTTCTAGATTCAGCCGAAACCCCCGCAAGTTGTGGAGACGCA13.225806451612903No Hit
TCTTGGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAG13.225806451612903No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC13.225806451612903No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT13.225806451612903No Hit
CTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCT13.225806451612903No Hit
TGTTTATTGAGTGAGGTTTGGTGCCCAGACCTCCCCGCCCCGAAACTCAC13.225806451612903No Hit
GTACTATTGTCGCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT13.225806451612903No Hit
GGGAAGCAGTCAGAGGACAGCTCACATGGGCCCTCACAGGTCATGAGCAG13.225806451612903No Hit
TGAGCAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACCGTGGTGA13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)