FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091135
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG138041.2651046845715699No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA128811.1805138685863803No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA127841.1716240428544589No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA106260.9738483322411984No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA102530.9396637446328823No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG86560.793302386964033No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT84790.7770807461954753No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG77530.7105445247380022No Hit
GCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC69130.6335604668533225No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC67840.6217379151067466No Hit
GAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTACAG60100.5508026046272918No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT59670.5468617540450998No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC57520.5271575011341402No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG56250.5155182447634802No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT54620.500579671626334No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG53980.4947142195970251No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC51870.47537655743789725No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT50800.4655702548263964No Hit
GTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAG49210.450998272441082No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC47150.43211884872174383No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT46750.4284529412034258No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA44780.41039834667570924No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGAT41000.37575552062760337No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA40870.37456410068415No Hit
GAATGAATGAGATACCCAATTACAGCAGACAAGAGAATAAGGCAACCAAA40340.3697067732223785No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT38150.34963592955958706No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC37860.34697814660880644No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA37680.3453284882255633No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG37540.344045420594152No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG37480.34349553446640424No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA37080.33982962694808616No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC35750.3276404844496786No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC35580.32608247375439336No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG33600.30793623153871885No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG32430.29721345204763844No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA31990.2931809537774886No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT30260.2773259037607629No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT30200.2767760176330152No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA30140.2762261315052675No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA29920.2742098823701925No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG29710.2722852809230755No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA29580.2710938609796221No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG29380.2692609072204631No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG29280.26834443034088357No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG28670.2627539213754485No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG28260.2589963661691725No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC27960.2562469355304339No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG25960.2379173979388435No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG25550.23415984273256746No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC25270.2315937074697448No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT25140.23040228752629144No Hit
CTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCA24960.22875262914304828No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT24730.22664473232001542No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG24500.2245368354969825No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT24490.22444518780902456No Hit
TGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCT24470.22426189243310865No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC24370.22334541555352913No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT23850.21857973577971562No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA23150.21216439762265898No Hit
GAATAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGTGCAT22790.20886508085617272No Hit
CTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCA22620.20730707016088754No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC22320.20455763952214895No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC21530.19731747217347073No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG21030.19273508777557313No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA20610.18888588488133914No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC20490.18778611262584372No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20490.18778611262584372No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20400.18696128343422216No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA20000.18329537591590409No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA19260.17651344700701563No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT19160.1755969701274361No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT19070.17477214093581456No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTAAGAGGAATCG18810.1723893010489078No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA18630.17073964266566466No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC17740.16258299843740692No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA17590.16120828311803764No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT17350.15900873860704678No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA17240.15800061403950932No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT17150.15717578484788775No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA17140.15708413715992978No Hit
GCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC17130.15699248947197186No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG17060.1563509556562662No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA16820.15415141114527534No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA16770.15369317270548555No Hit
CCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACAACAA16770.15369317270548555No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT16620.1523184573861163No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA15920.14590311922905966No Hit
GTTCATTGATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTC15690.14379522240602674No Hit
GAGAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACTTAC14650.13426386285839972No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT14480.13270585216311456No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG14400.13197267065945095No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG14100.1292232400207124No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTC14070.12894829695683852No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT14060.12885664926888057No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA14050.1287650015809226No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG13850.1269320478217636No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT13800.1264738093819738No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA13670.12528238943852044No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA13580.12445756024689887No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC13310.12198307267203418No Hit
ACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCA13220.1211582434804126No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC13180.12079165272858079No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA12870.11795057440188428No Hit
CAAGAGAATAATGGACATGATTCCAGAGACAACAAGGCAACCAAACGACT12800.11730904058617861No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA12630.11575102989089343No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA12570.11520114376314572No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG12510.114651257635398No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA12380.11345983769194463No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA12370.11336819000398668No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT12360.11327654231602871No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG12250.11226841774849125No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT11970.1097022824856686No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA11840.10851086254221522No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG11780.1079609764144675No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC11760.10777768103855159No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT11680.10704449953488797No Hit
GAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGA11350.10402012583227557No Hit
CACCAATACTCAGAAAAGGGAAAGTAAGAGGAATCGTTCTATTCTCAACA11340.10392847814431762No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC11310.10365353508044377No Hit
GCTCAGTTCATTGATGCTTAATGCTGGGCCTCTTGTCTTCTTTGCCCAAA11180.10246211513699038No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC11040.10117904750557906No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC11020.10099575212966314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA16950.0116.788591
GCGGACC256.461474E-6116.013244
GCAGGTA5050.0107.685552
AGCGAAA3300.0107.668741
CAGGTAC5300.0102.606043
GGTACTG5550.0100.597065
CAATCCC1600.099.689735145
CGAAAGC3900.091.1001363
GCGAAAG3900.091.1001362
GAGCGAA406.1120227E-790.6394961
GGCTTCG259.3861425E-487.00195145
GGTCAAA12800.084.408031
TGCGGAC353.435473E-582.866593
AGGTACT7550.076.8299564
GTCAAAT14150.075.839042
TATATTC27600.075.660813
ACTGATC7550.074.898918
AAATATA14900.074.45485
GAAAGCA4900.073.988044
TAATGGC406.659693E-572.508284
Produced by FastQC (version 0.11.5)