FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGCGTCCTATCACCATGAGCTACAACAAGGTAGAAGTTCAGAGCAT12.272727272727273No Hit
AAATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG12.272727272727273No Hit
CACCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGTTACATG12.272727272727273No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATGCTGGATCA12.272727272727273No Hit
GAGCCACACAAATGGGAAAAATACTATGTTCTTGAAATAGGAGACATGCT12.272727272727273No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG12.272727272727273No Hit
GTGGTAGTGAGTATTGACCGAATTTTAAGGGTGAGAGATCAAAGAGGGCA12.272727272727273No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTCGATGATG12.272727272727273No Hit
CCTATATTACCGTCTAGGGAAAAATTGATAGAATAGATTGCATACCTTCG12.272727272727273No Hit
GGGTATTGGAATCAAGGAACAAAGACTTAAGAGACATACCAACCCAGTTT12.272727272727273No Hit
GCCACAATTATTGAAACAGCAAATCCCTCATGATCTATCACTCTTCCTGT12.272727272727273No Hit
AAATAAGGCCTCTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATG12.272727272727273No Hit
GATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGACCA12.272727272727273No Hit
GGGGCTATGTTGACAAAATGACCGTCGACAACATCCACAGCTCTCTGCTG12.272727272727273No Hit
ATACCCAATCTTACCAGTCATGAAGTCATAATTAAAAGGGTGCTATAGTA12.272727272727273No Hit
GTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAA12.272727272727273No Hit
GCTGCACTGTTTCACGAGTGACCACCAGATCCGGAAAAACATGAACAACA12.272727272727273No Hit
ATATTCAACAGTCTATATGCGTCTCCACCAATTCAGGGGGTTTCGGCTGA12.272727272727273No Hit
TTTATAAAGATGCTCAACAGCATTATTCAAATCGGTACTGGAGACTATAT12.272727272727273No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTATTCCTGATTTG12.272727272727273No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA12.272727272727273No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAAATGAGAGATCTAATG12.272727272727273No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG12.272727272727273No Hit
CAGAGAACTCTTCTTTCTTTATACCGACAGAATAGAAGTCGCCCCTGGCA12.272727272727273No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA12.272727272727273No Hit
GCGGCGAGGGGCTACCCACCCGGGCCATGCAGAGCGCAGGGCCCAGTAGG12.272727272727273No Hit
CCATATGGCCATAATCAAACACCAGACCAGAGAATAAAGGACATGATTCC12.272727272727273No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT12.272727272727273No Hit
CCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCT12.272727272727273No Hit
AGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCCCCATG12.272727272727273No Hit
CCCTATTCTTCCCATTTTCTATGAGAAAAATATACCTTAAATCATAACTA12.272727272727273No Hit
GCAAAAAGACCTAATATATAATTACTAATGTCCAATTTCAGATAAGAAGC12.272727272727273No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12.272727272727273No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGCTA12.272727272727273No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGATCGCTTTCAGA12.272727272727273No Hit
CCCCAGTATTCAATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGA12.272727272727273No Hit
CCGCAGTTACTCTGATTCAGGTCAGACCGCCAATACCCCTATGTGTATAT12.272727272727273No Hit
ATTTTTGCCGTCTGAGTTCTACAATGGTCGAACAGATCTTCATGATCTCA12.272727272727273No Hit
CATTAGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCCTCTGGA12.272727272727273No Hit
CTCTTCACCCCCTTCTAGGCCAGACCCTCCACTGCAGGGGAGAAGGTTGC12.272727272727273No Hit
CGCCTGGTGAAGGCGAAACAAGCTGCTGTACGACACGAATTAGTTGCAAG12.272727272727273No Hit
CGGTCGGACTGCAGAAAGGCATCCCAGAGACTCAGACGGGCAAGTTCGTG12.272727272727273No Hit
ATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTTTATCAA12.272727272727273No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)