FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGGTCATCGTATTTAATGTCCGTGCTCATAATAGCTAGGCTCTCAT13.225806451612903No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG13.225806451612903No Hit
GGGCAAATAAATACAAAATCGAGAGAATAAAACATATGAGAGATCTAATG13.225806451612903No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC13.225806451612903No Hit
GAATTCTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGC13.225806451612903No Hit
CCCTGGAACTGAGAAGCAGATACTCGGCCATAAGGACCAGCAGGGGAGCA13.225806451612903No Hit
TATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACAACAGCATTGTT13.225806451612903No Hit
GGACAAAGAACATGAAGAGAACCAGCCAATTGAAGTGGGCACTCGGTGAA13.225806451612903No Hit
GAGTTTAAACAAGATGCATTAAGCAAAACACAGGGATCATTAATCAGGCA13.225806451612903No Hit
CATGAGCAAAAAGAAGTCCTATATAAATAAGACAGGGACATTTGAATTCA13.225806451612903No Hit
CACCGGAATGGATCCAAGAATGTGCTCTCAAATGCAAGGTTCAACACTTC13.225806451612903No Hit
GGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTGCAGGACCATACTGGCA13.225806451612903No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT13.225806451612903No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCA13.225806451612903No Hit
GGTGAACAGTATATGTAACACAACAGGGGTAGATAAGCCTAAATTTCTTC13.225806451612903No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTAGTCAGTGTTTGT13.225806451612903No Hit
ATGGTATTCTCACAGGAGGAATGCATGATCAACGCAATTAGTGGCCATCT13.225806451612903No Hit
CTGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTAACATGGTGG13.225806451612903No Hit
GCATAAAAGAACTGCGAGATCTAATGTCCCAGTCCAGCACTCGCGACCAA13.225806451612903No Hit
CCACCACTACGTCCCCTTGCCCAATTAGCCTTCTCTCCGTTTGGAAGATT13.225806451612903No Hit
TGCCGTTGTACTTTAACACAGCCACTGCCCCATTGTCTGGGCCAGAAATT13.225806451612903No Hit
GTATATGTAACACAACAGGCGTAGAGAAGACTAAATTTATTCCTGATTAT13.225806451612903No Hit
GGATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACT13.225806451612903No Hit
GCACCACATTCCCTTATACTGGAGATACTCCATACAGCCATGGAACAGGA13.225806451612903No Hit
CTCCGTCCCCTTGACCAATTATCACATTAGACTTCTCTCCTTTTGCAAGA13.225806451612903No Hit
CTCAAGTTGTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTA13.225806451612903No Hit
CATTAATCAGGCACTCCTCGATTGCTTCATATAGCCACCCAAGATCTAAG13.225806451612903No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGATATACTCACTAAGACCACTGT13.225806451612903No Hit
TAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTT13.225806451612903No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT13.225806451612903No Hit
ATTTATATAGGCATTCTTTTTGCTCATGTACATTCCCACTAACTTGAAGG13.225806451612903No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)