FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT60.3065917220235054No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA50.25549310168625444No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.2043944813490036No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT40.2043944813490036No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA40.2043944813490036No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC40.2043944813490036No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT30.1532958610117527No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.1532958610117527No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC30.1532958610117527No Hit
GTTTTATTATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGC30.1532958610117527No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG30.1532958610117527No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG30.1532958610117527No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT30.1532958610117527No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG30.1532958610117527No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG30.1532958610117527No Hit
TCCCTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCAGG30.1532958610117527No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG30.1532958610117527No Hit
TCTCTAGGTAATATATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATG30.1532958610117527No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG30.1532958610117527No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA30.1532958610117527No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.1532958610117527No Hit
TCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCATATTTTCACCGAG20.1021972406745018No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA20.1021972406745018No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG20.1021972406745018No Hit
ATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTTTGGAC20.1021972406745018No Hit
CCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTTTTAACTTGCC20.1021972406745018No Hit
GATGTGGGAGATCAATGGCCCTGAGTCAGTGCTAGTCAACACTTATCAAT20.1021972406745018No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGT20.1021972406745018No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC20.1021972406745018No Hit
CAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAA20.1021972406745018No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA20.1021972406745018No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT20.1021972406745018No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA20.1021972406745018No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT20.1021972406745018No Hit
CAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAA20.1021972406745018No Hit
GTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCAACGGCCCA20.1021972406745018No Hit
ATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCC20.1021972406745018No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA20.1021972406745018No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT20.1021972406745018No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC20.1021972406745018No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC20.1021972406745018No Hit
GATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCATTCC20.1021972406745018No Hit
CAACAAGTACAGTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAG20.1021972406745018No Hit
CCTCAAGAGTTGGTGCATGGGGTTCAGTCGCTGGTTTGCCCTATTGACAA20.1021972406745018No Hit
ACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCAT20.1021972406745018No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA20.1021972406745018No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG20.1021972406745018No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA20.1021972406745018No Hit
GGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTG20.1021972406745018No Hit
GTAAAATAGTTCCTCCTCATGCTTGCGATATGTTCAATCGGGGCAACATC20.1021972406745018No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCT20.1021972406745018No Hit
ATCAAAATCCTCGAATGTTCCTGGCGATGATTACATATATCACCAGAAAT20.1021972406745018No Hit
GAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATA20.1021972406745018No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT20.1021972406745018No Hit
ATTCAGTATCGAGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCA20.1021972406745018No Hit
GTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATCC20.1021972406745018No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.1021972406745018No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA20.1021972406745018No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG20.1021972406745018No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA20.1021972406745018No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.1021972406745018No Hit
TTTCAGTCTCTTGTCCCTAAGGCAACCAGAAGCCGGTACAGTGGATTCGT20.1021972406745018No Hit
GAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATC20.1021972406745018No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.1021972406745018No Hit
CCTGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCG20.1021972406745018No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC20.1021972406745018No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT20.1021972406745018No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT20.1021972406745018No Hit
GTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACGG20.1021972406745018No Hit
TCTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCAT20.1021972406745018No Hit
AACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACAT20.1021972406745018No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT20.1021972406745018No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA20.1021972406745018No Hit
GACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAGTTTTGATT20.1021972406745018No Hit
GTGCAACACTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAA20.1021972406745018No Hit
CCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGA20.1021972406745018No Hit
ATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAGATGTAC20.1021972406745018No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC20.1021972406745018No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG20.1021972406745018No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20.1021972406745018No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA20.1021972406745018No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG20.1021972406745018No Hit
CATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT20.1021972406745018No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20.1021972406745018No Hit
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC20.1021972406745018No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG20.1021972406745018No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.1021972406745018No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT20.1021972406745018No Hit
GTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGG20.1021972406745018No Hit
GCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACA20.1021972406745018No Hit
GAAATTTCACCATTACCTTCTCTTCCAGGACATACTTATGAGGATGTCAA20.1021972406745018No Hit
ACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACC20.1021972406745018No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT20.1021972406745018No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT20.1021972406745018No Hit
GTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAG20.1021972406745018No Hit
GGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTTCCTTGAAGAA20.1021972406745018No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20.1021972406745018No Hit
CAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAG20.1021972406745018No Hit
GAACTGAGAAGTTGACAATAACTTATTCGTCATCAATGATGTGGGAGATC20.1021972406745018No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT20.1021972406745018No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20.1021972406745018No Hit
GTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTCT20.1021972406745018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCTT100.006560914145.09
TCAGTCT100.006560914145.08
Produced by FastQC (version 0.11.5)