FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAATACTAAGAAAAGGGAAAGTGGAGGACAAACACAGAGACTGGTGC15.0No Hit
GTTTCAACCTTTCGACCTTTTCGAAATAAGTTTTATATACCTTAGGGTAA15.0No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC15.0No Hit
GCAGTAGGCAGGGGTTTTAGAGAGGCAGAGCAGGGAGCCCAAAGGGTAGT15.0No Hit
ATTCAATGGTCACAAGATCCCACAATGTTATACAACAAAATGGAATTTGA15.0No Hit
CTAATTCATGGCCATCAGAATTCTTTTTGTCGCTGTCTGGCAGTCAGTAA15.0No Hit
ATCCTCAACCCTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGT15.0No Hit
GTTTCTCCCAGTAGCCGGCGGAACAGGCAGTGTTTATATTGAAGTGTTGC15.0No Hit
TAGTAGCAGTGCCACAACTACTTCAGTGCATTTGATAGGAAGGAGTTGAA15.0No Hit
CTAGTGAAGTGTTTCCAGTAACACACGGTCCGTGAGGGTCGCAGATGGCA15.0No Hit
GATTTCTGAAGTGGACAGGGCCGAAGGGACCATGTTTCAACCTTTCGAGC15.0No Hit
GTATGTTTCTGCTCGCTTAAGTAACCTCTACATCCAACACGGGGCTCCAA15.0No Hit
CTATTAGTCCGAAAAGTAAATGAATTAAAAAGAGGGGGAAAAGTCATTGC15.0No Hit
CCCCTCTCCCTGGGCCCTCTTCCCCACCGCCAGGCCATTCCTTGTGTGGG15.0No Hit
GTAATTATCCCGGATGTGCCACCCTTTGTGTTTGGTCCCTCCTTGCTCCC15.0No Hit
TCCCAAGACCGTACTTCGCATGGTGCACCTGCCCATCCTCATCCCCGGAC15.0No Hit
CCCCATACCCGTTTCTCAGCCTGGGAGCCCGTGAGATGTGGGGAGCACAG15.0No Hit
GCCTGCGGTCACGGCGTCACTGGTAGATTTTGTCTGAGAGTTTTGACACT15.0No Hit
GGCCTGTAGCTCTGCTAGCACCTGCGTCCAAGCCCGGAGGGAATGGGGAT15.0No Hit
GGCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAA15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)