FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_F01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_F01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382724
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA12360.32294812972272446No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6600.17244803043446452No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC5860.15311294823423668No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA5040.13168758687722745No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC4060.10608166720665546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTC350.003565050762.141859
ATGGGCG400.006048431654.37412145
CTTATAA1004.6937203E-850.749176145
CGGTGAC450.00963524148.332559
CAAGGTA1351.0233634E-842.962265145
TAGAAAC3650.039.740963
AATACCT5350.037.958195
GAGTTAT1553.461173E-837.4187477
ATACCTA5600.036.263636
TAATACC5400.036.2636264
TTAATAC800.002031295836.2636263
ACCTATC5250.035.9041828
TTATAGT2650.035.5794074
CAATTCG850.002740098934.1170929
CCTATCA5600.033.6601689
TACCTAT5400.033.564277
TGTAATA6050.032.3675352
TATTACA5650.032.0917052
ATTGAGT1851.6337253E-731.3631364
TATAGTT2351.2514647E-930.8626615
Produced by FastQC (version 0.11.5)