FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_E12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_E12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTT12.7777777777777777No Hit
AGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAA12.7777777777777777No Hit
ATATAGAAGAGGAGACAGAAAGTGGATGAGCGAACTTATACGTTATGACA12.7777777777777777No Hit
TTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATG12.7777777777777777No Hit
ATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGT12.7777777777777777No Hit
CAAAAAACATATACCATATAATTAATCTTCACCAGAATGAAGACTCCAAA12.7777777777777777No Hit
CAACAAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTG12.7777777777777777No Hit
CTACAGGACATTGAAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAA12.7777777777777777No Hit
ATAAAAGTCCTGAGAGTTCTAATGTCGCACTCCCGCACTCGGGAGAAACA12.7777777777777777No Hit
GTAGATGAATAATCCAGAAACGAGAGAGTGGAAGTGACTATTGAAGGATT12.7777777777777777No Hit
CATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATG12.7777777777777777No Hit
CTCTTTGACAGAAGACTCGGCCACAATCATGCACTCAATCTGATTAAGAG12.7777777777777777No Hit
CTGTAGCGGCGTCTGGGTGCCGTAGTGACAAGAGACGGAAATGGTTCAAA12.7777777777777777No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCCGCCGAAA12.7777777777777777No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG12.7777777777777777No Hit
ATTATATTCCAGATGGCAAGAATACCAGAAATACGCAAGCGACTGTCGAA12.7777777777777777No Hit
CTTACTATGTTTCTAGCAGCGATAATCAAACTTTGGTCAACATCATCATT12.7777777777777777No Hit
GGTGCATGGGGTTCAGTAGCTGGTTTGCCCTATTGAAAAAGTTCCGATCG12.7777777777777777No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA12.7777777777777777No Hit
GTGCACACAAGCTCAACCCGATTGATGGACCACTACATTAGGATAATGAA12.7777777777777777No Hit
CCTCTCAGGACAGCAGCCTCCACCCCCGATGTGCCTTCCTCTGGCTCTTC12.7777777777777777No Hit
TTAAAACCGTTCTCAGATATGAAATGCTAGGGACACATGTCTAGACGATA12.7777777777777777No Hit
GAGTATTGGAGTAACAGTGATAAATAACAACATGATACACAATGACCTTG12.7777777777777777No Hit
GTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGAATCCACT12.7777777777777777No Hit
ATTAAAGGATGTAATGGAAGCAATGAACAAAGAGGAAATAGAGATAACCA12.7777777777777777No Hit
TCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAG12.7777777777777777No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA12.7777777777777777No Hit
GGTATGCACAAACAGACAGTGTTCTAGATGCTATGGATTACCTTGAAGAA12.7777777777777777No Hit
TCCATGCCCCATTTCATCTTGATCTTGGAGGTTCCATTGGTTCTCACATA12.7777777777777777No Hit
GGGTATGAAGAATTAACAATGGTTGGGAGAAGAGCAACATCTATTCTCAT12.7777777777777777No Hit
GGGTGTTCAGTTGGTGTCATCAGGCTGACCACTTGGCGGGTTTGGAGTAA12.7777777777777777No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA12.7777777777777777No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGCCACACAAATTCAGACGA12.7777777777777777No Hit
AAACAAGGTTTCTCCCAGTAGCCGGCGGAACAGCCAGTGTTTATATTGCA12.7777777777777777No Hit
GTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGCTA12.7777777777777777No Hit
GTATAAGTTGGGGAATCAAGATGATAATAATAGACAGACCTGTGCTTTGG12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)