FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_E05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_E05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG47.547169811320755No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA23.7735849056603774No Hit
CCATAGGGGAGCCCCACAAATTCAGACGAGAAGATCATTTGAGTTAAAGC11.8867924528301887No Hit
AATTAAATTAATTAAATTTCCCGTCTTAGTTCTTCAATTTACGAACAGAT11.8867924528301887No Hit
AGCAGGTACTGATCCAAAATGGATGACTTTGTGCGACAATGCGTCAATCC11.8867924528301887No Hit
GTAATACAACAATAAACTACATATTGGGTAGTATAGACATCATAACAATA11.8867924528301887No Hit
ATGTGTTAAACGTCATAATATTCAATGCTTTTTGCTGGAACATGTGATGG11.8867924528301887No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGTCCATAATCAAAAA11.8867924528301887No Hit
GGTCTGCAGACACTGATCATCTAATTCCTATGCAAATAAAAACACTAAAC11.8867924528301887No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA11.8867924528301887No Hit
TAATAATCAGCATCAAATAACTGATCAATCTTGAAACAATCGAAAATGAT11.8867924528301887No Hit
ATGCACACATTTGGAAGTTTGTTTCATCTATTCGGATTTACATTTCCTCT11.8867924528301887No Hit
TAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCATTATACTGCAGA11.8867924528301887No Hit
GGGCCCGAGAATGTTAAAAACGTTACAGGAAATTCGTCGTTCTATGAAAA11.8867924528301887No Hit
GGGAAACATCAGTTCTCTATCCAACCCTGTACTGCATCTGCTATTGTGAG11.8867924528301887No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC11.8867924528301887No Hit
TCATTGCCGAGCAGCCCGCCGCAGGCCCTTGGCCCGCTGCGGGCAACGCG11.8867924528301887No Hit
GGAGAAATCCTCTCAGGACAGCAGCCTCACCCCCAGAGGTGCCTTCATCT11.8867924528301887No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA11.8867924528301887No Hit
ATGCTTATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACA11.8867924528301887No Hit
TTGTAAGACGTATTGACCAGCTAACCATCATCATCTATTTTATTTTAATT11.8867924528301887No Hit
GTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGA11.8867924528301887No Hit
GATCATTAATAAGGCACTCATCGCTTGCTTCATATAGCCCCCCCAGAACG11.8867924528301887No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGCCAAGTTAAATTCATTATTTT11.8867924528301887No Hit
CAACTACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACA11.8867924528301887No Hit
TCGGAAACATGGCCAATCGGAGAGTCACCCAGGGGAGTGGAGGAAGGCTC11.8867924528301887No Hit
CCTCCATACAGCCATGGAACAGGAACATGATACCACATGATGAACAGATG11.8867924528301887No Hit
CGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTGTGGCCAGGGTAT11.8867924528301887No Hit
GCTGAGACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGT11.8867924528301887No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT11.8867924528301887No Hit
AAATATAATAGTCCAGCTGTGCTTTCCAATGCACCACACGCTAATGTAAA11.8867924528301887No Hit
AAGCTCAGCCAATCCTACATTAATCATAAAGGCAAAGAAGGCCTCGTCCT11.8867924528301887No Hit
CCGATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.8867924528301887No Hit
AGTCGAGACTAATTGATGGCCATCCGAATTCTTTTGGTGGCTGTCTGGCT11.8867924528301887No Hit
GAACATTGATCCTTAATGCTGTGCCATATATCTTCACATCATTGAGGAAA11.8867924528301887No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACAGCAGGAAGGCAAGAGAA11.8867924528301887No Hit
CTCTTATCCATCTCAGAGACATCTCCTTTCTTGGCGTCATGTCGGTCAGT11.8867924528301887No Hit
ACATAAGTCCATAATCAAAAAGAACACATAATTAATGCAAGAGAAGAACA11.8867924528301887No Hit
GAATGAAGTGGATGATGGCAATGAGATGGAAAGGATGCAGGTGCATTGCC11.8867924528301887No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGCTACTCACTAAGACCACTGT11.8867924528301887No Hit
GTTCTCTTCATGTTCTTTGTCCTTGGGATCTTCTCTTCATTTTCAATGTC11.8867924528301887No Hit
GTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGTTTCTCCCAGTAG11.8867924528301887No Hit
GTTAGGGGCGATCTGAACTTTGTCAATAGGGCAAACCAGCGACTGAACCC11.8867924528301887No Hit
TCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGC11.8867924528301887No Hit
AGCCGGAACTTTGCCCGCTGATGGCAAAGAGGCCCCGCAGGCATTCTGAG11.8867924528301887No Hit
GTATGGAGAGGGAACTTCACCAATAGGAAAGCTCATTAGGGTTCGATATT11.8867924528301887No Hit
TTATTAAACAATTCGACACTAAATGATGACAATACGAATTCATTTGCTCG11.8867924528301887No Hit
AAACAAGGGTAGATAAACTAACTCAAGGTCGCCAGACTTATGATTGGACA11.8867924528301887No Hit
GGGCAGTACATAGCCGAAACCATAAATAATCATGTAACGTATAGCACCAA11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATG50.0145.02
GATATGA50.0145.03
AACTGCT50.0145.09
ATGAACT50.0145.06
TATGAAC50.0145.05
GAACTGC50.0145.08
ATATGAA50.0145.04
ATCATAA50.0145.0145
TGAACTG50.0145.07
CCGATAT50.0145.01
Produced by FastQC (version 0.11.5)