FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_E05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_E05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694092
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6550.054.242691
GCGAAAG7150.049.690862
CGAAAGC7350.049.3252333
AAGCGTA2100.044.8859565
AGCGTAG2500.037.7042056
GCGTAGC3100.030.402137
TACGCGA1306.480592E-427.8877282
GTATTAA10150.026.431521
TAAGCGT4303.6379788E-1223.6072854
TTATACG1550.001807831323.3897064
CAATTCG2202.2306083E-523.0673489
GTATATA23850.022.8012851
TATACTT19100.022.3977875
GTAAGCG5400.021.483883
TACTTAC15200.020.9860867
TATATAC27400.020.9056173
TTAGACG2455.112298E-520.7165974
TATACAG15950.020.4568275
ATATACT17350.020.0598744
CATATAT18950.019.8966791
Produced by FastQC (version 0.11.5)