FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAGAGAACAAGCGGCTCATCAGTCAAGAGAGAGGAAGAGGTGCTT12.0408163265306123No Hit
ATACATAGGTTACAACTACACTAATAGACAGACTTAATAATTAAATATAG12.0408163265306123No Hit
ATGATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTG12.0408163265306123No Hit
ACATGGGCCTCGACACTTGGCCTATCGCAGTCCTCAAGAGCATGTCTCCT12.0408163265306123No Hit
GATCTAATGTCGAACTCCCGCACTCGCTAGATACGCACTAAGCAGACTGT12.0408163265306123No Hit
GGGGCTAATGGATAGGGCTGTTAAGATATTTTAGTAGTTTCTAAAGGAGG12.0408163265306123No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATG12.0408163265306123No Hit
CCACTATATGATGCAATCAAATGCATGAAGACCTTCTTTGGCTGGAAAGA12.0408163265306123No Hit
TCCCAGGACCCTGGGAAAATGACCTGAGCCAAAGGCACAAACTTAACAAC12.0408163265306123No Hit
CTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGGATAAGAGT12.0408163265306123No Hit
TCATTCCACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTC12.0408163265306123No Hit
CCCTAGGACTATGCCTTGGTGGAACGTATGTCAACGGAAAAAGTTTGAGT12.0408163265306123No Hit
CTGGCAAATGTTGTGAGAATGATGATGACTAATTCACAAGACACAGAGAG12.0408163265306123No Hit
CTCAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAG12.0408163265306123No Hit
GCTGGCTGCACCCCCCCAGGGGGGTTACTGCCAGCCTGGCGGGGGCGCAC12.0408163265306123No Hit
ATCTCTCTCTGTCAAATAAATAAATAAAATATTAAAAACAAAAACATTTG12.0408163265306123No Hit
TGATTGCATAATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCA12.0408163265306123No Hit
CAAGCAGGCAGGCAGGATTTATGTGCAACTGATCCCATAAGAATGAGTGC12.0408163265306123No Hit
GATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGAATTTT12.0408163265306123No Hit
GCCGTAGACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTT12.0408163265306123No Hit
CAATATGGTAATGCACTCTACTTCTTCTTGGCCCATTCCTAATCAACCAA12.0408163265306123No Hit
CAGTAAGTGCATCTAAACTTTCTTTAAGGGAGAACTTCATTTGGAATTTG12.0408163265306123No Hit
GTTGTAAAACCAGCCAGACTTGCTGAAGCACCTGACTGGTAAGATGGTCT12.0408163265306123No Hit
TGTTAGGACAACAACTCTGCTCAAACTGATCTATAGATTTAATGCAGTCT12.0408163265306123No Hit
GTAGTACTGCGTACTACAGTAGTAGTCAGTACTACTGTAGACTGAGTGGC12.0408163265306123No Hit
TTGTTAGCAGGGACGGTGTTTTCCCGCACGAAGTGGGCGCACGGATAGTA12.0408163265306123No Hit
AATAAATACAGTCTTTTTAAAAAAAATTGTATTTATTTATTGGAAAGGGA12.0408163265306123No Hit
AACTAACTCAAGGTCGCCAGACTTATTATTGGACATTAAACAGAAATCAA12.0408163265306123No Hit
GCACAAACCATACCGTGCAGGCCTCCATAACTACCACCAGATTCCAAATT12.0408163265306123No Hit
TCTGTATTAAGTAGACCTGCAGTTGGACCCTGCTGCTGTAAAATAGTTCC12.0408163265306123No Hit
GGGGGTAATGGATAGGGCTGTTAAGATATTTTAGTAGTTTCTATTGGAGG12.0408163265306123No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT12.0408163265306123No Hit
ATCCTATGCAGCAACTTTTAAGACATTAACAGAACGGTGCGAAATTGCTT12.0408163265306123No Hit
TCTCAATTTTCTTCTTTGTTGATTCATTGAAGTACTTCAGGTCAATGCTA12.0408163265306123No Hit
GGTTCATTCAATTTGGCTGTAGAGATGCTCTGCAGCAGCACGAGGAGGAC12.0408163265306123No Hit
GTGTCTAATGGATAGGGCTGTTAAGATATTTTAGTAGTTTCTAAAGGAGG12.0408163265306123No Hit
CCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTTGACATCCTCAT12.0408163265306123No Hit
GCTATTGTTCCAACTCCTTTCACNGCAGCACCTGCGGCACNAGACCTTCN12.0408163265306123No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC12.0408163265306123No Hit
AGTGGGAATTGCCATTGCCCAGGCCCTGTACTGAGCCACACTGGGAAATG12.0408163265306123No Hit
AGTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTC12.0408163265306123No Hit
ATATCAAACAACCCCAAAATATCAGTGGTTTAACAGAGTAGATATTTATA12.0408163265306123No Hit
GGGGGAGACAGATTCCCACTGAGGACCAAGCCGTAGACAGATGAAAGGCA12.0408163265306123No Hit
GTGTCTAATGGTTAGGGCTGTTAAGATATTTTAGTAGTTTCTAAAGGAGG12.0408163265306123No Hit
AATTCAACCTGTCTCTTGTGTTTGAACATGAGGAAAAAAAATGTTTCGGG12.0408163265306123No Hit
TTCCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGG12.0408163265306123No Hit
ACCTAGGGGCCCCATGTGCTCCATTTTATTATTTTTTAAGATATTTTATT12.0408163265306123No Hit
AGTTGATTCTAATGTGAAACTAGAGTTGAAACCCATTGGAATAGATGTTT12.0408163265306123No Hit
ATATTAGACCCAGGTGACAGTCCTGAGATCTAAATTGTTGTGTTTCTATG12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)