FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA171.2345679012345678No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG151.0893246187363834No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA110.7988380537400145No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT100.7262164124909223No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC100.7262164124909223No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG90.6535947712418301No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA90.6535947712418301No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC90.6535947712418301No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG80.5809731299927379No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG70.5083514887436456No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT70.5083514887436456No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC70.5083514887436456No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC60.4357298474945534No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA50.36310820624546114No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG50.36310820624546114No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC50.36310820624546114No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG50.36310820624546114No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG50.36310820624546114No Hit
GGTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGA50.36310820624546114No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG50.36310820624546114No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT50.36310820624546114No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC50.36310820624546114No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA50.36310820624546114No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT40.29048656499636893No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG40.29048656499636893No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT40.29048656499636893No Hit
GGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGA40.29048656499636893No Hit
ACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA40.29048656499636893No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.29048656499636893No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG40.29048656499636893No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA40.29048656499636893No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGG30.2178649237472767No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT30.2178649237472767No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT30.2178649237472767No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC30.2178649237472767No Hit
GGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTG30.2178649237472767No Hit
GACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGAT30.2178649237472767No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT30.2178649237472767No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA30.2178649237472767No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.2178649237472767No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA30.2178649237472767No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC30.2178649237472767No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC30.2178649237472767No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA30.2178649237472767No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC30.2178649237472767No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG30.2178649237472767No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA30.2178649237472767No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA30.2178649237472767No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA20.14524328249818447No Hit
CCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTA20.14524328249818447No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG20.14524328249818447No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20.14524328249818447No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC20.14524328249818447No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20.14524328249818447No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC20.14524328249818447No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA20.14524328249818447No Hit
GCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTT20.14524328249818447No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA20.14524328249818447No Hit
AAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATT20.14524328249818447No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20.14524328249818447No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCC20.14524328249818447No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT20.14524328249818447No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT20.14524328249818447No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG20.14524328249818447No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT20.14524328249818447No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT20.14524328249818447No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT20.14524328249818447No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG20.14524328249818447No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA20.14524328249818447No Hit
GCCTTGTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCGTTTG20.14524328249818447No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT20.14524328249818447No Hit
AATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCA20.14524328249818447No Hit
GAAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAA20.14524328249818447No Hit
CTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTG20.14524328249818447No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG20.14524328249818447No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA20.14524328249818447No Hit
TCATCCATTAGCTCCCATTTTAAGCAGACTTCAGGAATGTGAAGATTCCG20.14524328249818447No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT20.14524328249818447No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA20.14524328249818447No Hit
CAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTC20.14524328249818447No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG20.14524328249818447No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTGG20.14524328249818447No Hit
AGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAA20.14524328249818447No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT20.14524328249818447No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG20.14524328249818447No Hit
AAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAG20.14524328249818447No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT20.14524328249818447No Hit
GCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA20.14524328249818447No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC20.14524328249818447No Hit
CTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGC20.14524328249818447No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA20.14524328249818447No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20.14524328249818447No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC20.14524328249818447No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20.14524328249818447No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG20.14524328249818447No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20.14524328249818447No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT20.14524328249818447No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)