FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTAGGCTGCACACTGCTCTGGCCTGCGGATGCGTTTTGTTGATTGGT13.8461538461538463No Hit
ACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGACCAAGAA13.8461538461538463No Hit
CTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGACCAAGAAGATGGTCA13.8461538461538463No Hit
GCTCGCGTCGTCAAGGGTTTATTACTCTGTGTGGGCCAGCTCCTCTCCAG13.8461538461538463No Hit
TTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAG13.8461538461538463No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTA13.8461538461538463No Hit
CAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATA13.8461538461538463No Hit
CACAAGAACACAAACAGCCTAATCACATGTAAAGTGGCCGAACAACACAG13.8461538461538463No Hit
GAGCAAGAGGTTCCAGGAGACTCTGAGTAAAGGATCCGACAACCACGCCC13.8461538461538463No Hit
CTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAA13.8461538461538463No Hit
ATCCTGAGCATGGCACCTATAATGTTCTCAAACAAAATGGCAAGACTAGG13.8461538461538463No Hit
GTATATTGTCTTGGTGTATTTCCTTTGTCCAAGATTCAGTATCGAGACTC13.8461538461538463No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG13.8461538461538463No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA13.8461538461538463No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC13.8461538461538463No Hit
AACCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAA13.8461538461538463No Hit
CTCTTTTAGTAGAGGCCTTTTGTTCTCTATTTTCACTGTTCGTGAGAGAT13.8461538461538463No Hit
TATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGT13.8461538461538463No Hit
GAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAG13.8461538461538463No Hit
AAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGT13.8461538461538463No Hit
CTCTTATCCCTCTCAGCGACATCTCAGTGCTTGGGGTCATGTCGGGCAGT13.8461538461538463No Hit
CAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCATAT13.8461538461538463No Hit
ACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATC13.8461538461538463No Hit
AAGCTAAGTACGGTCTTGGGAGTCTCGAAACTGAAACATCGACAAAAGAA13.8461538461538463No Hit
GGTCCAATGTCCCTGTGGTTGTTATATAGGAGGTGTTGTTGCTCATGTTG13.8461538461538463No Hit
CAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCA13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)