FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGG12.380952380952381No Hit
TGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATACTGACA12.380952380952381No Hit
GAATACACGCAGGAGTGGACAGATTCTACAGGACCTGCAAGTTAGTGGCA12.380952380952381No Hit
CAGGAAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTAT12.380952380952381No Hit
CAAAAATACTCAGAAAAGGGAAAGTGGAAGAAAAACACAGAGACTCTTTC12.380952380952381No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCCAAAAGCTTGA12.380952380952381No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG12.380952380952381No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGAACAGGAACAGGATACAC12.380952380952381No Hit
GGCTGCGTTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTATGTATT12.380952380952381No Hit
GCCCCCCAGCACGGAGATGTCGCTGAGAGGGATAAGCGTCAGCTAAATGG12.380952380952381No Hit
GCAGATCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTT12.380952380952381No Hit
CGCAGATTCCATGCTTTTGGCTGGACCAGGGGCTGGCATTACCACAGCAT12.380952380952381No Hit
GGGGCCGGCTCATGCCTCGGCAAAATATAAGAGGCCCGCTTTGCGGGCGA12.380952380952381No Hit
GTAGGTGGATGGTGAATGCAACATAGCACTAGGACTTATTTCCCTTTATC12.380952380952381No Hit
GTGAGGCTGTTCCCGCTATTAGAAGCGGCATTATTTTCGCAATTTTCTTC12.380952380952381No Hit
TAGTTGAATAGCTTAGTGACACCTCCTTGGACCCATGGAACGTTATTTCT12.380952380952381No Hit
AGGTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCAT12.380952380952381No Hit
AATTTAGGCTTCTCTACCCCTGTTGTGTTACATATACTGTTCACCACTGT12.380952380952381No Hit
CTCACAGACAGATGGCTACTACCACCAATCCACTAATCAGGCATGAAAAC12.380952380952381No Hit
GTCAGTAAGTATGCTAGAGTCCCGGTTTCGTTTGAATACCCACACTACGT12.380952380952381No Hit
GTGCAGGGCGAGTGGTTAGATAATGCTGGGTGGGTACAGGTCGACGGCGT12.380952380952381No Hit
GGATAGAACTTGATGAAATAGGAGAAGATGTTGCCCCGATTGAACATATC12.380952380952381No Hit
ATACAAGCAGGAGTGGAGAGATTCTACAGGACCGGCAAGTTAGTGGGATT12.380952380952381No Hit
GCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAGGAAGG12.380952380952381No Hit
AGTAAGGATGGTGGACATCCTTAGACAGAATCCAACTGAGGAACAAGCCG12.380952380952381No Hit
GATCCGTCCAGCCTCGAAGTCGACCCTGGCATCCATCCGGGCCCTAGACA12.380952380952381No Hit
ATGTAATGCAATCAAAGAACAAAGAGCAAATAGAGATAACACCCCACTTT12.380952380952381No Hit
TTCCAAGAGAGATGCTAATGGCTCTGCTGACACTGCTGCTCTTCTTACTA12.380952380952381No Hit
GCAAAAAGCATGGACTATGATGCCGTGGAAACGACACATTCATGGATTCA12.380952380952381No Hit
GCCCTGCGTCCGTGCTCGTCACCACGTAGCCATGGAGAAGCAGGAACGGG12.380952380952381No Hit
GGTATGAAGAATTCACAATGGTTGGGAGCAGAGCCACAGCTATTCTCAGA12.380952380952381No Hit
TATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCAT12.380952380952381No Hit
CGAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCATATA12.380952380952381No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG12.380952380952381No Hit
CAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTT12.380952380952381No Hit
CCTGTGGCATATCCGCAAGCGATGTGCATAGAATGGATTGGGTGAGGCCC12.380952380952381No Hit
GGCTCGCACTACGGCAAAGGCTATGTAACCGCTGGCGGGATAGAGTGACC12.380952380952381No Hit
GTGGACCATATGGCCATAATCAAAAATTACACATCAGGAAGTCAAGAGAA12.380952380952381No Hit
GTGTTGCGATAGCCCTTCTTTTTAACTTGCCTCTCTCTGCATCTTTGGTC12.380952380952381No Hit
GCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTGGATCAATCTCCT12.380952380952381No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC12.380952380952381No Hit
GTTTTATATAACTTAGGGTAATGAACGGTAAGTGTTCTTGGGCCATTCCT12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)