FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593266
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG41490.2604084942501754No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG39780.24967582312055864No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA37730.23680917059674908No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT36640.22996787730360152No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA34570.2169756964624865No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC34280.21515553586155733No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC33050.2074355443472716No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT31830.19977831699163856No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG31540.1979581563907094No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG28620.17963102206411233No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT27840.17473541768919942No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA27530.1727897287709648No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA27020.1695887566796756No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT26960.1692121717277592No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG26210.16450485982880447No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT25150.15785185901161514No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC24900.1562827550452969No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA24390.15308178295400768No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA23430.14705642372334563No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG22530.14140764944459996No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT22180.13921090389175442No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT21750.13651204506968706No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT21330.1338759504062724No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT21070.13224408228130144No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20140.12640701552659755No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA20030.12571660978141755No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT19820.12439856244971022No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG19620.12314327927665561No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19490.12232734521417014No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA19000.11925190144018638No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT18830.11818491074309No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC18650.11705515588734085No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA18560.11649027845946629No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA18350.11517223112775896No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT17950.11266166478164977No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG17840.11197125903646973No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG17640.11071597586341515No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT17180.10782882456538959No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA17020.10682459802694591No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT16740.10506720158466948No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA16420.10305874850778214No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT16400.10293322019047668No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT16370.10274492771451847No Hit
CACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG16270.10211728612799119No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC16060.10079923879628386No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG16010.1004854180030202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA4750.0108.39011
AGCGAGC554.1654857E-1092.28539145
CAGGTAC1600.090.640283
TCGACGG259.3826617E-487.01194145
ACCGTCT259.389683E-486.995558
GCAGGTA1850.082.311182
GCTATAC1050.075.965193
GGTACTG1950.074.371525
GCACCGT300.001934659172.512236
CACCGTC300.001936346172.49637
GTAGAAA7500.068.6449052
GACAGTA2900.064.996687
CAATTCG5550.062.6994979
AACTCAC350.003564509362.1533363
TTTAAAC5850.060.7367363
AGGTACT2400.060.4268574
AGCGAAA1154.0017767E-1156.7504771
CTGCTAC400.00604825854.38246145
CAAGGTA2300.050.4322059
CGAAAGC1301.3096724E-1050.2007753
Produced by FastQC (version 0.11.5)