FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA80.2609262883235486No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG80.2609262883235486No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC80.2609262883235486No Hit
TATTTGGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACA70.228310502283105No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.19569471624266144No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG50.16307893020221786No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC50.16307893020221786No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT50.16307893020221786No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT50.16307893020221786No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC50.16307893020221786No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT40.1304631441617743No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA40.1304631441617743No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT40.1304631441617743No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG40.1304631441617743No Hit
CCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATT40.1304631441617743No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT40.1304631441617743No Hit
AATCCATAGCGATAAAAAAAGCTTGTGAATTCAAATGTCCCTGTCTTATT40.1304631441617743No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT40.1304631441617743No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT40.1304631441617743No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC40.1304631441617743No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT40.1304631441617743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGAGT100.006750369145.08
ATGAGTC100.006750369145.09
Produced by FastQC (version 0.11.5)