FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375677
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA100540.7308401608807882No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT75160.546349179349513No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT53840.3913709395446751No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT45330.32951048829049256No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG36860.2679408029646494No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC35260.2563101658310781No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC33780.24555182648252463No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG31070.22585243483753814No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG30650.22279939258997572No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG29900.21734753143361416No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG26550.19299588493519917No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT25800.1875440237788376No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG25260.18361868374625728No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT24590.1787483544465743No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23950.17409609959314576No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC23860.17344187625438237No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA23660.171988046612686No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG23330.1695892277038869No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC23160.16835347250844493No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG23150.16828078102636013No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC22450.16319237728042266No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA22200.16137509022830215No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA21680.15759513315989146No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG21390.15548708017943166No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA21070.15316095275271738No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG20700.15047136791557902No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA20610.14981714457681564No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20580.14959907013056117No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG20580.14959907013056117No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC20530.14923561272013708No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG20340.14785447456052547No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA19530.141966464511655No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA18980.13796843299698983No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC18730.13615114594486932No Hit
AAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAG18720.13607845446278452No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG16880.12270322175917747No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG16640.12095862618914177No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA16200.11776020097740968No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT15970.11608829688945878No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG15690.11405293539108381No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG15640.11368947798065969No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG15590.11332602057023561No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA15360.11165411648228472No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT15320.11136335055394544No Hit
ACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTG15200.1104910527689276No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG15070.10954606350182493No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA14810.10765608496761958No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC14810.10765608496761958No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG14760.10729262755719547No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC14630.1063476382900928No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA14410.10474842568422675No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT14300.10394881938129372No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA13960.10147730899040981No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT13860.10075039416956161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT100.0070877266145.02342145
TCGCGCG100.0070877266145.02342145
GCTATAC600.0120.857243
AGTAGAA8550.0112.804191
GCAGGTA3600.0112.8000952
TGTAGTG352.7596434E-7103.58815145
GGTACTG3950.0102.8051455
CAGGTAC4300.094.437283
AGCGAGC1000.087.014046145
AGGTACT5550.075.7807544
GTAGAAA12900.075.3249742
CGAAAGC1500.072.514343
TGCGGGC406.658746E-572.51171145
TTATAGG6300.066.7616651
GACAGTA5350.066.398117
AGCGAAA1650.065.924531
CTACACT1051.6370905E-1162.1551484
CTCCGAC350.003564507262.15289145
GCAGGGT1102.5465852E-1159.329924
GCGAAAG1900.057.2481652
Produced by FastQC (version 0.11.5)