FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315432
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG52220.3969798514860517No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA48440.3682440445420212No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT45480.3457419311678597No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA33600.25542939505804935No Hit
CTATAAAACATAATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGA19810.1505969141696416No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA18990.14436322059977255No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA17200.13075552366066812No Hit
ATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGAATATACTGGGGT16650.12657438772965837No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG16340.12421774747763473No Hit
TCCTTAGGGTGATCCCAGGGCTTTTGTCATTCTGTCTTCTCCATTCCCAC15780.1199605908933339No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA15460.11752792998801914No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC15270.11608353757548852No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT14650.11137025707144117No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT13510.10270390259625735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC300.001936087772.498024145
CGAAAGC7150.069.9844063
GGATAGC1500.067.68541
TAAGACG350.003562843762.1600654
AACCGGT400.006051962354.373516145
GTAAGGT2450.053.280063
GGATCTA553.214978E-452.741871
GCGAAAG10300.048.5814132
CGCTCCG450.00964084648.332012145
AGCGAAA10700.046.765281
CGAAGAC803.735903E-545.311264145
GATAGCA2250.045.1236042
CATACAT18750.043.6921468
TACAGGA2250.041.900494
TACATAC20000.040.611246
ATATATG16650.040.071153
GTGGTCC3100.039.756988
GGGCGTC750.001483755838.665617
CGAATTG750.001483755838.665619
ATAGATA3900.037.1784748
Produced by FastQC (version 0.11.5)