FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297722
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG51710.398467468379206No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA48060.37034125953016134No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT45010.3468385370672609No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA33280.2564493782181392No Hit
CTATAAAACATAATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGA19550.15064859808186962No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA18830.14510041441849642No Hit
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA17040.13130701336649914No Hit
ATGTAATAGCATCACATTTCTTAAGTTAATGTTGGGGAATATACTGGGGT16450.12676058508679056No Hit
GTCCTAAGTTGTAAGCAGTGTTTTGACTGTTGTAGGTGGCGCCTGCTGTG16200.12483413242589707No Hit
TCCTTAGGGTGATCCCAGGGCTTTTGTCATTCTGTCTTCTCCATTCCCAC15660.12067299467836717No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA15380.11851536769816648No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC15180.1169742055694517No Hit
GTTGTAGGTGGCGCCTGCTGTGAGCATCTGTACGAATTTCGAGTTTTTGT14480.11158013811894997No Hit
CCCCAAGTGTTAACCAACAGATGATAAATCTTATGTACCATGGAGTATGT13400.10325786262389018No Hit
GTTTTATAGTATCTGTTGAGAAAAGAAGTGACTGAAGAGAAAATACAAAA12980.10002142215358914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC6400.078.181083
CGATCCA300.00193420272.515786
ACGATCC350.003563668862.1563845
GTAAGGT2700.059.0869333
GGATCTA753.755431E-758.0148661
CCTAGCG502.0098376E-458.003677145
AACCGGT553.218351E-452.73062145
GGGTAAG3500.051.7989851
CACGCGT858.913503E-751.1876146
ACGCGTC858.9277273E-751.175787
GCGAAAG10000.050.035892
GGATAGC1950.048.345721
AGCGAAA9950.048.1027761
CATACAT16950.045.766348
GGTAAGG3750.044.4763452
GTGGTCC3950.044.0500378
TACATAC17650.043.9614076
CATACCG1053.8192866E-641.4375885
GGGCGTC700.001056828141.428017
ATATATG19300.040.5787773
Produced by FastQC (version 0.11.5)