FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366866
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC19740.14441796050234623No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA18710.13688247421473648No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT18010.13176126994160364No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG17220.125981625119068No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA15850.11595869675593658No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15640.11442233547399672No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA15330.11215437358160932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2550.073.9476851
AGTAGAA7150.058.831971
CGAAAGC3800.049.6191563
GCGAAAG4450.042.3714182
CCTTATA7250.041.0114862
GACAGTA4450.040.726857
GTAGAAA11600.037.513251
TTATACT8300.036.6970254
CAGCTCG4500.035.4414069
CAATTCG6350.035.3906639
TAGGGTG1102.4326115E-432.9637764
CCCTTAT9750.031.2418061
GGCGGTT1453.4933673E-529.9974277
AGGTCGT4100.028.3006086
GTCTAAG10650.027.239671
GGTCGTT4000.027.1851677
CAGGTAC6250.026.6874733
CAAGGTA5500.026.3613769
TATATTC8700.025.8405693
TAGAAAC20250.025.7853
Produced by FastQC (version 0.11.5)