FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA25.88235294117647No Hit
CCTCTGATGTCAGTATTCTTGCCCCCACTCCATTTGGGAAAACAACTTCC12.941176470588235No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGAGAGGAAGGAG12.941176470588235No Hit
CCCTCAGAGCACAGTACACAGAGCTACCTGTGAGCGGCTGGCAGGGGGGA12.941176470588235No Hit
GGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATGGATGCTCTGAA12.941176470588235No Hit
GCTATTCAACTGCTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGC12.941176470588235No Hit
GTCTTACGCATTACTTGATTTTGTACGAAACACTCCAAGAGACAAGTTAA12.941176470588235No Hit
GGATGTAATGGAATCAATGAACACAGAGGAAATAGAGATAACAACCCACT12.941176470588235No Hit
AAAAAAGGTACTAATAAGCACAGTATAGATATCAAATAGAATCATTCCCA12.941176470588235No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA12.941176470588235No Hit
GCACTGGGCCAGACCTGTTTAGAAATGGGAAAGATTTTGAATTTTAAAAA12.941176470588235No Hit
CACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTC12.941176470588235No Hit
CAAGAGAATAATGGACATGGCCCAGCATTAAGCATCAATGAACTGAGCCT12.941176470588235No Hit
CTGAAAGCTTTACATTGTCTAATATCTGAAGAATGACAGATTCACGCACA12.941176470588235No Hit
TCATTGCAGCAAATATCATTGGGATCTTGCAACTGATATTGTGGATTACT12.941176470588235No Hit
CAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATAGGCCATGTT12.941176470588235No Hit
AGGAAGGTCTCATTTGAGAAATGATACTGATGTGGTGAACTTTGTAAGTA12.941176470588235No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT12.941176470588235No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACAATG12.941176470588235No Hit
CCCTAAGTGCCTGAACAATGAGAAGCAATATTCTAGATTCAGCCGAAAAC12.941176470588235No Hit
CTATTGAAGCTCCGATTTGAGATAAATGAAGGAAGAGACCGAATCATGGC12.941176470588235No Hit
CCCTGTCAGCAGCTTCCGGCTGTTCAGAAACTCTTCACGGGGAGGCTTGA12.941176470588235No Hit
GTGTAGAGGATGGCTTGGAAAGGGAGGGACTGGCCGTACAAAAAGTTCAC12.941176470588235No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG12.941176470588235No Hit
GAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGA12.941176470588235No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA12.941176470588235No Hit
ATTGATGGCCATCCGAATTATTTTGGTCGCTGTCTGGCTGTCAGTAAGTA12.941176470588235No Hit
AACTTCCACCATGCCAATAGCTCTAGGAACAACTCCCCAGCAGCACAAGC12.941176470588235No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAACTCCTTAAAAGGAAGAGGCAAA12.941176470588235No Hit
TCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGA12.941176470588235No Hit
TCATTACAAAGAAACCAACTGCGAGACAAGCCTTATACATATTAAAGGCA12.941176470588235No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG12.941176470588235No Hit
GTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTATTTGTTGATTCATTG12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)