FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376741
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2000.068.8904951
TGTAGCG604.9420213E-448.34072
ATCGGAC450.00963410348.34073
GCGCTAT657.3386094E-444.614089
CAATTCG3900.044.6140759
CGAAAGC3800.040.071893
CCTTATA4900.038.4752462
GTAGAAA10650.036.7688331
AGTAGCG800.002035358236.2555245
GCGAAAG4350.035.0078771
TTATAGG5750.034.0511361
TATACGT1102.4344467E-432.9595644
AGTAGAA9150.032.493651
ATATAAC1703.0092287E-629.8574893
GGTCGTT2500.028.999157
GACAGTA4750.028.9991477
TTTAAAC7800.026.9592323
ATATTCA7800.026.9592324
TATATTC9150.026.9439953
GTATTAC8500.026.4471241
Produced by FastQC (version 0.11.5)