FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAATACTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGA27.6923076923076925No Hit
GTCGAGTTTAAAGTCTACGTCTTTTTCTTAACACCTAGGTTGTCTTTCTG13.8461538461538463No Hit
ATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGC13.8461538461538463No Hit
GGTTTTACAACTCTTGTCAGAGGCTCAGACACTCTACGGAAAGCTTACAT13.8461538461538463No Hit
ATCCGGGATGACGCCACCAGTGCTCTGGCCGTCCAGTGTGAGCAGGCTCG13.8461538461538463No Hit
GTTTTAGAAGAGGCGTGAGTTTGTCATTGGTCTGCGTTGTGGTTTCATTG13.8461538461538463No Hit
ATAGTAAGTGAGTAGGTGGGAAAGGCAAAGACATATAAATGGTGTAGAAA13.8461538461538463No Hit
CAGTTCAATAAGGAGATCCTGCTGGGTCGTGGCTTCACCTTTTGGCAGTG13.8461538461538463No Hit
ATTGTGAATAATGCTCCTATGAACACAGGTGTACAAATAACCTTTTGAAA13.8461538461538463No Hit
GGCCTGGTCTGGCGCACAGGCTCTCCGCAGTCTGACCGATGGGCTTCCGT13.8461538461538463No Hit
GATCATCAGTCAAGAAAGAAGAATAACTGCTAACGGGCAACCACCAAACA13.8461538461538463No Hit
AGACAGTGGCATCTGCACTTGGGTAGTAGCAGTAGAAGTGGGAAGAAGTC13.8461538461538463No Hit
GTTTGGTGCTTTCACTATGAGAGCAAAATCGTCGGATGCTTGGAGACCAT13.8461538461538463No Hit
GTTCCATCGCCTTTGCCGTAGTGCTCGCCAGCACCATTCTGGTGTCAGGC13.8461538461538463No Hit
GGTGGAGGGAGAGTCCATGCCGAGAGGCTTTTGCTCCCTCCCTGTCCCCA13.8461538461538463No Hit
GCTGAGGCGCTGCGCCTTGGCATTCAGATCTGCCTCCCGGGGGGCCAACT13.8461538461538463No Hit
CAAATATGAGATTGAATTTAAATTAAAACAAAATACAATGCCTCCAAACA13.8461538461538463No Hit
GTGTTCGACTCTTCATTATTTTACTCTCCAACATGTGGCCTTTCCCTAGT13.8461538461538463No Hit
GTCTACGGCTTGTTGCTCAGTTGGATTCTGTCTAAGGATGTCCCCCATCC13.8461538461538463No Hit
TCTCTACACAGTGCCGAGATGAAGGGCATCAAGATCAAGGTCCAGGCCCC13.8461538461538463No Hit
CTTCTTAACGTACTGAGCCAACCAGTCGCCGAAACTGAAGTAAAATATGA13.8461538461538463No Hit
AAATAATCTATTCACTAGAGCTAAGAATTACATTTGGAATAATAACTCTT13.8461538461538463No Hit
GCTAAAAACAACATGGGAACTAAAGCAAGCTGGCTCCTGCTGATCCTCAC13.8461538461538463No Hit
GTATACCATAAATCAAATCTCACTCATTAAAAATATAATGCTATAGACCA13.8461538461538463No Hit
CGCCAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGA13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)