FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT50.22727272727272727No Hit
GTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTCAAGAGTTGGT50.22727272727272727No Hit
GGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATT50.22727272727272727No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT50.22727272727272727No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC50.22727272727272727No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG40.18181818181818182No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT40.18181818181818182No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT40.18181818181818182No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG40.18181818181818182No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG40.18181818181818182No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG40.18181818181818182No Hit
GAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATCAGGGT30.13636363636363638No Hit
GATGTACACTCCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTT30.13636363636363638No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.13636363636363638No Hit
TGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCG30.13636363636363638No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.13636363636363638No Hit
CTTATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTT30.13636363636363638No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC30.13636363636363638No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA30.13636363636363638No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT30.13636363636363638No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG30.13636363636363638No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.13636363636363638No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA30.13636363636363638No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT30.13636363636363638No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA30.13636363636363638No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA30.13636363636363638No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30.13636363636363638No Hit
GATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTGA30.13636363636363638No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT30.13636363636363638No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT30.13636363636363638No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA30.13636363636363638No Hit
CGCAAAGAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTAT30.13636363636363638No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC30.13636363636363638No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.13636363636363638No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT30.13636363636363638No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC30.13636363636363638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACAGA100.0066203587145.0145
ATCATAT100.0066203587145.0145
Produced by FastQC (version 0.11.5)