FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACCCAGCTGGCTAGAGATCTGGGCTCTGGCTCATCAATGTCAGACTGT18.333333333333332No Hit
GAGAGAGTGGTAGTGAGTATTGCCCGCTTTTTAAGGGTGAGCGATCAAAG18.333333333333332No Hit
GCGAGGGAAAAATTTCTCGAATAGATTGCAGCACTTGTGGAACATCTCTT18.333333333333332No Hit
GGGAAATCCAGAGTGTGAATAACTCTCCACAGCAAGCTCATGGTCCTACA18.333333333333332No Hit
GCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTT18.333333333333332No Hit
CTCTTCAATTGTTCATCAAAGACTACAGATACACATATAGGTGCCATAGG18.333333333333332No Hit
GTCTTCATGCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTG18.333333333333332No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAAAATACGGAATCTT18.333333333333332No Hit
GGTAATGAACTGTACTTGTTGTTGGGCCATTCCTATTCCACCATGTTACG18.333333333333332No Hit
AGAGAGAAGGCAGAGAATTCAGAGCTCTTTACTGAATCTGAGTATGAATT18.333333333333332No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTATATA18.333333333333332No Hit
ATGCCAAGGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGAT18.333333333333332No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)