FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771588
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAACTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTG87751.137264965240517No Hit
CTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCA41340.5357781614021991No Hit
ATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCA40730.5278723878546582No Hit
CTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACAGGGAGTTT29540.38284680425304696No Hit
GTTTCTATGTGCAGACATGGGTAGACCGTCTTATCTCTGTTTCCCCATCT28270.3663872429327569No Hit
AAATAGGGGTGGCGAAGGAACTCGTGTTGCAAGCCAACTCTGATTAACTG28240.3659984343976319No Hit
GTGTATACTCTGCCTGAACGTAATAATAGCAGATATATTGGTGAACAAGG27870.36120312913109065No Hit
GCACAAAGGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCCTATTG27630.35809266085009095No Hit
TACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAG27600.35770385231496604No Hit
GGGTAAGCAGAAGCCAGGGTATCAGCCAGAGACACGCGGGGACTGGTGGG27020.3501868873025501No Hit
CCCTCAGGCATCTCCTGCTCAGCCACAGTTTCTATGTGCAGACATGGGTA26610.3448731706558422No Hit
GTTTAAAGTGGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCCAG26540.34396595074055064No Hit
GCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACA25710.33320891460209334No Hit
GTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCCACAGTTTCTATGTG24900.32271108415371935No Hit
GAGTATACACTGGGTATTTTAAAATATGGATGATGGATTGGTATGCGTAT24820.3216742613933861No Hit
GACATGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTAC24630.3192118073375947No Hit
ATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCATTCTG23980.3107876224098871No Hit
GCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAA23370.30288184886234626No Hit
GCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAG23130.29977138058134656No Hit
GTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACAGGGAG23080.29912336635613823No Hit
CCCTCAACATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCT22890.29666091230034686No Hit
GTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAAC22800.2954944866949719No Hit
AGCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGCAC22440.29082878427347236No Hit
GGTCGAGCTCATGTGACAATAAGAAGAAGAAAAACAGCAGTAATAACTGT22340.2895327558230558No Hit
GTCCTAAGCGAGGACTAAGAGAGAATGAAGGCCACTGTCCCAGGTCACCC22050.28577427331684785No Hit
TGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCA21290.27592445709368213No Hit
TTTCAGCACAAAGGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCC20170.26140893844901686No Hit
GTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTT19570.25363276774651755No Hit
TAATAACTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGC19430.25181832791593445No Hit
GTATCAGCCAGAGACACGCGGGGACTGGTGGGCATCTCCTCCCTCTCCCT19200.24883746247997637No Hit
GGCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCAC18770.24326454014318524No Hit
CATGTGACAATAAGAAGAAGAAAAACAGCAGTAATAACTGTTATCATTTG18370.23808042634151905No Hit
AAAGCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGC18300.2371732064262275No Hit
GTACTGTTGGTGTACCCATTTTGCAGATGGGGAAACAGAGATAAGACGGT18240.23639558935597751No Hit
GTGCAGACATGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATG17430.22589775890760355No Hit
GCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGCACA17070.22123205648610397No Hit
CAACATACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTT16490.21371509147368803No Hit
GAAGAAAAACAGCAGTAATAACTGTTATCATTTGCCATGCTCCGGGCTCC16450.21319668009352144No Hit
GGCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTGTCACAACATA16340.21177104879806322No Hit
ACCCATGTCTGCACATAGAAACTGTGGCTGAGCAGGAGATGCCTGAGGGA16180.20969740327739672No Hit
GTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAGACACGCGGG16130.20904938905218848No Hit
CTATTGAAGAGCTGAGATCCCAAGGCTCCCACATCCCTGGCCAAGTTGGA15880.20580931792614712No Hit
AAAGCAGGTCGAGCTCATGTGACAATAAGAAGAAGAAAAACAGCAGTAAT15770.20438368663068893No Hit
GCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTGTCACAACATAC15690.20334686387035567No Hit
CCCTGAGACTGTCACAACATACCTACTCCTACCTGGAGGGAAAGTACTGT15640.2026988496451474No Hit
GTATCAGGCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTGTCAC15480.20062520412448093No Hit
TCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTTC15250.19764433868852288No Hit
GTCCCAGGTCACCCAGCTAGGAAGCGCTGTCAGGACTTGACGCAGGCCCA14730.19090499074635686No Hit
AGCGAAAGCAGGTCGAGCTCATGTGACAATAAGAAGAAGAAAAACAGCAG14430.18701690539510724No Hit
CTCCTGCTCAGCCACAGTTTCTATGTGCAGACATGGGTAGACCGTCTTAT14200.1840360399591492No Hit
TCTCCTCCCTCTCCCTCAACATGCTCACCTGGGCGTCCAGGACCCTCCCT14080.18248080581864934No Hit
GTATTTAAGACTCTGTTTGTTTGTTCATTTGTTTGTTTTTTAGATTCCAC14050.18209199728352438No Hit
GTCACAACATACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCC13980.1811847773682328No Hit
AGCGAAAGCAGGGGGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTT13920.18040716029798287No Hit
GGATATACACAGTTAATGAGTCCTGCCAAGTGCAGAGAAAGCACTTCCCT13820.1791111318475663No Hit
GAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCC13800.17885192615748302No Hit
CATAGAAACTGTGGCTGAGCAGGAGATGCCTGAGGGAGGGTCCTGGACGC13630.1766486777917749No Hit
GGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACACCAA13610.17638947210169156No Hit
ATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTG13430.1740566208909418No Hit
GTCTTGGGCCTGATACTGGCTCTGCCACTCACTCGTTGTGTGACTTGGGG13310.17250138675044194No Hit
GGTATGTTGTGACAGTCTCAGGGCAGGCTCCTCGGCCAGACAGTCTTGGG13230.1714645639901087No Hit
GCATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAG13140.17029813838473382No Hit
GTTAATGAGTCCTGCCAAGTGCAGAGAAAGCACTTCCCTATCAATAATCG13130.17016853553969216No Hit
AAATAAGACGGCCAAGTTTCAGAACAAGAGACACTTAAGGATGGCAAAGA13090.16965012415952554No Hit
GCTTAGGACTGTGTATTTTTTATTTTCAAAAGCCTAAAAGCACGTTTGCC13080.1695205213144839No Hit
GCCCTGAGACTGTCACAACATACCTACTCCTACCTGGAGGGAAAGTACTG13070.16939091846944224No Hit
GAGTTTAAAGTGGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCC13000.16848369855415066No Hit
GCAGAAGCCAGGGTATCAGCCAGAGACACGCGGGGACTGGTGGGCATCTC12880.1669284644136508No Hit
GAGATAAGACGGTCTACCCATGTCTGCACATAGAAACTGTGGCTGAGCAG12790.16576203880827592No Hit
GGCATCTCCTGCTCAGCCACAGTTTCTATGTGCAGACATGGGTAGACCGT12760.16537323027315096No Hit
GTCTCAGGGCAGGCTCCTCGGCCAGACAGTCTTGGGCCTGATACTGGCTC12550.16265157052727622No Hit
CATGAAAGAGAGTCTTCAAGATATAATATTACAAAGACAAAGCACCCATA12530.1623923648371929No Hit
ATTTAAGACTCTGTTTGTTTGTTCATTTGTTTGTTTTTTAGATTCCACAT12410.16083713069669306No Hit
GAATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGCAG12390.16057792500660975No Hit
GCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGT12340.15992991078140145No Hit
GAGTAGGTATGTTGTGACAGTCTCAGGGCAGGCTCCTCGGCCAGACAGTC12290.1592818965561932No Hit
CATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCC12130.15720825103552674No Hit
GTCTACCCATGTCTGCACATAGAAACTGTGGCTGAGCAGGAGATGCCTGA12110.1569490453454434No Hit
CCCCAAGTCACACAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGAC11890.154097782754527No Hit
ATTTAATACCCTCTCTCTCAGGGAAGTGAAGCCTGGCGAGCCATCCCAGC11860.15370897421940205No Hit
CTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAGATGGGG11540.14956168317806912No Hit
GCCAGAGACACGCGGGGACTGGTGGGCATCTCCTCCCTCTCCCTCAACAT11530.14943208033302746No Hit
GGCTTACCCCAAGTCACACAACGAGTGAGTGGCAGAGCCAGTATCAGGCC11450.14839525757269426No Hit
CAACAGTACTTTCCCTCCAGGTAGGAGTAGGTATGTTGTGACAGTCTCAG11420.14800644903756927No Hit
GTGTATATCCTGCCCAGTCAAAGGCTTTGGCTTCTCCGAGGTAGAGAGGA11360.14722883196731934No Hit
CATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCCCAGCTGGG11230.14554399498177784No Hit
GGGCAGCATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGC10960.14204471816565317No Hit
CATGGGTAGACCGTCTTATCTCTGTTTCCCCATCTGCAAAATGGGTACAC10950.14191511532061152No Hit
ATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCC10820.14023027833507No Hit
GAGAATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGAAGGGGC10710.13880464703961182No Hit
GTACTTTCCCTCCAGGTAGGAGTAGGTATGTTGTGACAGTCTCAGGGCAG10680.13841583850448685No Hit
CCTGAGATTCCTGGGCAAGCGGAAGGGGCAGCATTCTGGGTGTTGGGCTG10620.13763822143423693No Hit
CTCCGGGCTCCATGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAG10470.13569417875861212No Hit
CACAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGACTGTCTGGCCG10450.1354349730685288No Hit
TGTCTGCACATAGAAACTGTGGCTGAGCAGGAGATGCCTGAGGGAGGGTC10370.13439815030819557No Hit
CCCCAACCCCACGCACTTACTGTGAGTTGTTCCTCTACTGGTGTCCCTGA10350.13413894461811227No Hit
GCAGTAATAACTGTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCA10280.13323172470282066No Hit
CAATAAGAAGAAGAAAAACAGCAGTAATAACTGTTATCATTTGCCATGCT10150.13154688771727915No Hit
CGCGGGGACTGGTGGGCATCTCCTCCCTCTCCCTCAACATGCTCACCTGG10150.13154688771727915No Hit
CTGTTTCCCCATCTGCAAAATGGGTACACCAACAGTACTTTCCCTCCAGG10070.13051006495694595No Hit
GTCCAGCACAGAGCTCAATCTCGTGACCTTGAGATCACGGCCTGAACTGA10050.1302508592668626No Hit
CTTCAATAGTGATGGGGATCCAACCCCACCTACTGCTGATAAAGGAGAGG9990.12947324219661271No Hit
TCCCAGCACAGCAGGGAAATGCTGATCCTCCAAATCCTTCTGGGCTAGCA9990.12947324219661271No Hit
GTTATCATTTGCCATGCTCCGGGCTCCATGCTGAGTCATTGCAGTTCATC9980.12934363935157106No Hit
CCGTAGAAGCTCCTGAAGAATGTCTTTCAGTCTCTGTCTTACTGGACCAC9920.1285660222813211No Hit
GAATAAAACTGTCAAAAAATAAAGAAAGTGACTTAGAGCAGGATCCATAA9900.1283068165912378No Hit
ATTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAGAC9810.1271403909858629No Hit
GGGACAGAGAATCCTATTGTGAAGGCACCTGAGATTCCTGGGCAAGCGGA9800.12701078814082126No Hit
GATTTATGGAATGGAAACCGCACAGGACTTAGGTTCAAATTCCAACTTCA9780.12675158245073795No Hit
GGTAAGCAGAAGCCAGGGTATCAGCCAGAGACACGCGGGGACTGGTGGGC9740.12623317107057136No Hit
ATATATTGGTGAACAAGGCAAACGTGCTTTTAGGCTTTTGAAAATAAAAA9620.1246779369300715No Hit
GTCACACAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGACTGTCTG9590.12428912839494652No Hit
TTCTGGGTGTTGGGCTGGGTAAGCAGAAGCCAGGGTATCAGCCAGAGACA9570.12402992270486321No Hit
CCAATATATCTGCTATTATTACGTTCAGGCAGAGTATACACTGGGTATTT9540.12364111416973825No Hit
GTATTAAATGGCTGGCTTTGGAAGCCACGCCACTCCTCTGCTTTGTGCCT9480.12286349709948832No Hit
CTCCCTCAACATGCTCACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTC9440.1223450857193217No Hit
GCCAGTATCAGGCCCAAGACTGTCTGGCCGAGGAGCCTGCCCTGAGACTG9220.11949382312840531No Hit
GTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCAT9150.11858660321311375No Hit
CACCTGGGCGTCCAGGACCCTCCCTCAGGCATCTCCTGCTCAGCCACAGT9110.11806819183294712No Hit
GTCTTGAGGGCTGTAAATTGAAGAACTCCCTGAGGCCACAACGAGTTCAG8930.11573534062219734No Hit
CAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCCCAG8920.11560573777715569No Hit
CTCTTTCATGAGTGTGTGTTGCTTTTAAAATAATAAAAGACAGCATGGAA8920.11560573777715569No Hit
AGGTAGGAGTAGGTATGTTGTGACAGTCTCAGGGCAGGCTCCTCGGCCAG8860.11482812070690576No Hit
GATATACACAGTTAATGAGTCCTGCCAAGTGCAGAGAAAGCACTTCCCTA8750.11340248941144755No Hit
TCTCCCAGCTGGGGAAACCGAGGCACAGGGAGTTTAAAGTGGCTTACCCC8740.11327288656640591No Hit
TGCTGAGTCATTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGT8630.1118472552709477No Hit
GCCACAGTTTCTATGTGCAGACATGGGTAGACCGTCTTATCTCTGTTTCC8610.1115880495808644No Hit
CACTTAAGGATGGCAAAGATTGCCAGGCACAAAGCAGAGGAGTGGCGTGG8350.1082183756097814No Hit
TCATAATCCCCTTTATTTCACATTCCTCCTGCCACTGGCAACCACCAGCT8320.10782956707465641No Hit
GGCTTGCAGCCAAGTTTTCTTTTCATTTTTTTCTTTTCAGCACAAAGGGC8240.1067927443143232No Hit
AATGAAAACCAGCTGCAGGGACAGAGAATCCTATTGTGAAGGCACCTGAG8230.10666314146928155No Hit
TCTCAGGGCAGGCTCCTCGGCCAGACAGTCTTGGGCCTGATACTGGCTCT8180.10601512724407326No Hit
AAGTACTGTTAGTGTTTCCATCTCCCAGCTGGGGAAACCGAGGCACAGGG8170.10588552439903161No Hit
ACCTACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAGA8090.10484870163869836No Hit
CAACGAGTGAGTGGCAGAGCCAGTATCAGGCCCAAGACTGTCTGGCCGAG8080.1047190987936567No Hit
CAAAGGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCCTATTGTGA8010.10381187887836514No Hit
CTGCAAAATGGGTACACCAACAGTACTTTCCCTCCAGGTAGGAGTAGGTA7990.10355267318828185No Hit
CCTCTAGGCTGTTGACCCCATGCTGCCCTCCTGCCGTGACTCAGCACCCA7980.10342307034324016No Hit
GTATACACTGGGTATTTTAAAATATGGATGATGGATTGGTATGCGTATCT7970.10329346749819851No Hit
TTCCTGGGCAAGCGGAAGGGGCAGCATTCTGGGTGTTGGGCTGGGTAAGC7930.10277505611803191No Hit
TTGCAGTTCATCTGTCCAGCAGGCTAAGTACTGTTAGTGTTTCCATCTCC7900.10238624758290694No Hit
GGGTACACCAACAGTACTTTCCCTCCAGGTAGGAGTAGGTATGTTGTGAC7870.10199743904778197No Hit
ACTCCTACCTGGAGGGAAAGTACTGTTGGTGTACCCATTTTGCAGATGGG7780.1008310134424071No Hit
GGGCTTGAATGAAAACCAGCTGCAGGGACAGAGAATCCTATTGTGAAGGC7730.10018299921719881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4700.0115.7161
GAGTGCG259.3876943E-486.995889
GCGAAAG6450.084.314732
CGAAAGC6550.083.027473
GACGACT353.435376E-582.86396145
GAGCGAA755.638867E-1177.3497241
ATAAAAC3300.076.905253
GATATCA2200.075.80662
ATATCAA2250.074.122013
TAGCACT502.3037264E-672.5106664
CTAGCAC502.3037264E-672.5106663
CGCTGCG300.001934942472.50597145
GGAGTGC300.001935937772.496578
TAAAACT3350.071.428414
AAAACTG3650.065.557585
TAGGGGT6600.064.820144
CCCATCG451.1932003E-464.449745145
GGATATC2600.064.148211
ATAGGGG6750.063.379693
AATAGGG6750.063.379692
Produced by FastQC (version 0.11.5)