FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542865
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.071.491661
CAATTCG5150.045.047849
CGAAAGC5750.044.1383253
GCGAAAG6400.039.655532
GTATTAC10200.033.4128341
TATATTC10400.027.1923623
AGGTCGT5500.026.3683536
GGTCGTT6150.025.9345537
TTACATC12950.024.637614
TACTCCG2651.3749559E-724.6270455
TATTACA16500.024.1709882
CCTTATA9650.024.045752
ATATTCA11800.023.3516314
TACATCA13500.023.0967245
TATAGGG3203.382229E-822.6603012
TTATACT12950.021.8378814
ATTACAT16350.021.7317163
TAGTACG2704.440557E-621.4853234
AGTAGAA11950.021.2381081
TATACTG14050.021.1603665
Produced by FastQC (version 0.11.5)