FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432185
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33670.23509532637194216No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA25330.17686262598756447No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA23450.16373583021746493No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC18850.1316170746097746No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG18620.13001113682939006No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT18240.1273578483226678No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG17310.1208642738193739No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT15010.10480489601552873No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14380.10040602296491026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5300.067.0386661
AGTAGAA15300.041.705791
TATATTC13000.036.254343
ATATTCA14550.032.8905334
GGTCGTT8400.032.7969637
GTAGAAA20100.032.466572
CGAAAGC11550.032.016823
CAATTCG15650.031.5009659
GCGAAAG12000.030.8161872
TAGAAAC22850.030.7804893
TCAATAT14950.030.551238
CCTTATA9500.029.766722
AAGGTCG15200.029.5717779
GTATTAG6350.028.5477221
TATATAC13250.028.4562343
ATATATT16650.028.3066922
AATATAT17300.028.0823761
CGTAAAC1050.00770504327.6223553
AGGTCGT10000.027.5532976
ATACGAG1100.00966731126.3667934
Produced by FastQC (version 0.11.5)