FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413936
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33390.23614930237295043No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA25020.1769528465220491No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA23230.16429315046791368No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC18650.13190130246347784No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG18440.13041608672528318No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT18070.1277992780437021No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG17190.12157551685507688No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT14870.10516741917597401No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14280.10099467019723665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4350.066.684741
AGTAGAA13950.044.18761
CAATTCG14650.042.0628559
CGAAAGC9200.036.2598273
TATATTC12150.033.4246983
GGTCGTT7000.033.141297
ACAATTC19550.032.6327258
GTAGAAA19600.031.449852
GCGAAAG10750.029.6824632
TTAAACA22750.029.6453974
TAGAAAC22750.029.007863
AAGGTCG13350.028.7814089
AACAATT22800.028.6170677
AGGTCGT8950.028.359646
TTTAAAC26600.027.8082873
TATATCG1050.00769932327.6265342
TCAATAT14100.026.2221648
ATATTCA13750.025.8433654
GTATATA16450.025.1283911
TAAACAA28000.024.6048835
Produced by FastQC (version 0.11.5)