FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578822
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4050.069.8295441
GCGAAAG6350.045.6774832
CGAAAGC6400.044.187613
CAATTCG4700.043.189669
GTATTAC8150.030.2517811
GTTACGC2901.2732926E-1130.005383
TATACTG16500.027.6867835
GTCTAAG6900.027.3246061
CCTTATA10050.025.9748082
GGATAGC4600.025.2219125
TACGCAG3501.2914825E-1024.86165
TATTACA12950.024.0776772
AGTAGAA12100.023.9721
TTATACT14500.022.5040364
CTATACT8400.022.44454
TACATCA12150.022.082155
ATATGCG3304.685353E-821.9736375
GTAATAC9950.021.1344433
TACCTAT9300.021.0474937
TAATACC13650.020.7184
Produced by FastQC (version 0.11.5)