FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610661
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT22540.13994254532766362No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA20450.12696650629772496No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA18360.11399046726778632No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC18260.11336960415630601No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA18100.11237622317793751No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA18000.1117553600664572No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG16320.10132485979358785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG5100.059.7055939
AGCGAAA3250.049.08431
CCTTATA8450.041.1857832
GACAGTA5750.037.8258977
AGTAGAA11450.037.3636931
CGAAAGC4900.035.5122342
CTTAACG6050.034.757291
TAACGAC6250.033.6419263
TTTAAAC9850.032.3876383
GTAGAAA13600.030.9208852
ACAATTC10200.030.5635788
GTATTAC13650.030.2792761
TTATAGG5250.029.004361
GCGAAAG5800.028.754321
ACGACTG7600.028.620065
TAGAAAC18400.028.3711873
TAGGCCG1050.007705338827.622343145
TACATCA14700.027.6206285
CTAGTAC2104.9871414E-727.6206263
ATATTCA9000.027.3887569
Produced by FastQC (version 0.11.5)