FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTGAGAGATATAATGTCGCAGTCCCGCACTCGCGAGATA12.941176470588235No Hit
AACCAAAGCTCTGTGAGTCCATCGGAGTTATCTCCAGGAAACCTCACGCA12.941176470588235No Hit
CCCATATACACAGGAAACAGCAAAACACAATCCTCAGAAACGGTCCAGTC12.941176470588235No Hit
CTATTTCCTCTTTGTTCATTGATTACATTACATCCTTTAAGAAATCTATT12.941176470588235No Hit
AGGCAGTCTTTGGGAATGCGGAGTTAGGAAATGGTGGACCATAGAGTACT12.941176470588235No Hit
ATACAATTGATGAGGTAGCAGAAATCGGGACTTTAATACAACATCAACTT12.941176470588235No Hit
ATGCAGGGTCCCCGCCCCGCTCAGAGCCTGTGACGGGGCTGATGCTGACT12.941176470588235No Hit
CTACAATGGAGTAACAGATCTTAATGATCTCAGAGAACTCTTCAATATTC12.941176470588235No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT12.941176470588235No Hit
GAAAAAGACCTGATATCTATAAAGATGACTGACATTTGAATGGATGAACT12.941176470588235No Hit
TTACTCATATCATGATTTGGCATTACACCCTGAATGATGCAAAATATCAG12.941176470588235No Hit
TGAAATATCTATATTTAAAGTGTGTTCTTTAAGAGGTGGGTTGTCACTGT12.941176470588235No Hit
GCTCAGGATGTTTCTGAACCACTCGCGTTGATTTCTTGTGATATATGTAA12.941176470588235No Hit
GGCAGATGGTACATGCAATGAGAACTATTGGGACTCATCCTAGCTCCAGT12.941176470588235No Hit
CCATACAGCCATGGAACAGGAACAGGAAACACAAAGGACACAGTAAACGA12.941176470588235No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG12.941176470588235No Hit
GGAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGG12.941176470588235No Hit
GTACCTCCAAACCAAGGCCTCCACAACGACAGCCTGGAACACCGCTCCCA12.941176470588235No Hit
AAAAAACCCCAGAGAAAACAGTTCCTGCTGCATTATTGTATGCCCGCTGT12.941176470588235No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG12.941176470588235No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATAAAAAAGTACACAACAG12.941176470588235No Hit
GTGAATGAAAACATGTGAATCTGTGTCTTCTAAGATTTTATTTTCTTGGC12.941176470588235No Hit
GCAATATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTT12.941176470588235No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCACTTGGGTTAAGTGCAAC12.941176470588235No Hit
GGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGACCC12.941176470588235No Hit
GAATAAAAGCACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12.941176470588235No Hit
ATAAAAAAAATACAAAATTAACAGAATTAAACATATAATATCACTAAATA12.941176470588235No Hit
GTCTTTGCACATGGCTCATCTGCTTTCAGCCTGGAATCATTTGATTCCCT12.941176470588235No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGCAGAGAAGATCATTT12.941176470588235No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA12.941176470588235No Hit
CAGAAAAAGTAGACTTTGATGACTGCAAAGATGTTGGAGACCTTAAACAG12.941176470588235No Hit
CTCAATGAGTGACATCGAAGCCATGGCGTCTCAAGGCACCAAACGATCAT12.941176470588235No Hit
GTCGGGCAGATTTCCGATCTTTCCCTGCACATTCACGATCGAATCATTTC12.941176470588235No Hit
GAGTTATTCAATGCTGGAAAAAATCTTCATCATCACAGACAACTCAAATT12.941176470588235No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)