FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAG22.0618556701030926No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC22.0618556701030926No Hit
CTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCC11.0309278350515463No Hit
TTGCAGTGTTTGGACTTGAATTTGTGGGACATTAGGTCAAAGTGCCTTTT11.0309278350515463No Hit
GCTTAGCCTTGGTCAGGGATTAGGGTGAGGGATAGCTTTCTAACAGGTTA11.0309278350515463No Hit
TCCCCCACAGACAATAAAATTGCTCCCGGGCCAACTGGCCATATCCTGGG11.0309278350515463No Hit
CTTGGGAAGTAACCCAGCTTTCTTTCTGTGGTACAGTTTTGGGCGGGACT11.0309278350515463No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC11.0309278350515463No Hit
GGATGAGGACTCGTGGGTTCCGCACAAGGGCCCGGGCAATGGCCAGACAC11.0309278350515463No Hit
AGAAAAAGTAGACTTTGATGACTGCAAAGATGTTGGAGACCTTAAACAGT11.0309278350515463No Hit
CTATCTGATATTCCAGATTCTGGTTGAAAGACGCCCACGGTCGATTCGAG11.0309278350515463No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11.0309278350515463No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT11.0309278350515463No Hit
ACACAGAGGGGGCCCCTAGACACCTATCTAAGAGAACCTTCCACAGAGAC11.0309278350515463No Hit
CTCCCTAAGAAGTAAGTTAAACGCAATGAACTCAGAATTTTACAATACCT11.0309278350515463No Hit
CCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATG11.0309278350515463No Hit
CCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTG11.0309278350515463No Hit
CGATTGATGGACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACAA11.0309278350515463No Hit
GGTGCGAGCAGTGAAACTATTCGAGAAGCTCTCGAGAGAAGTAACGTGCC11.0309278350515463No Hit
CACGTACAGTTCATTACCCTAAGGTATATAAACCTTATTTCGAAAGGGTC11.0309278350515463No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC11.0309278350515463No Hit
GGCCGAGCGCAAGGCAGCCGCAACGGCAGGACTCGCACTGAGCCCGCGTG11.0309278350515463No Hit
TCATTCCTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTAATTGG11.0309278350515463No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT11.0309278350515463No Hit
GCATAAATGTTATTTGTTCGAAACTATTATCTGTCGCTTTCAATCTGTGC11.0309278350515463No Hit
GTAGAAACAAGGCTATAGTTAATTGAAAATTCATTGTGCAAGGAACAAAG11.0309278350515463No Hit
GACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGG11.0309278350515463No Hit
AGATGGCTGTGGTGCTGCTGGCCAACCTGGCTCAGGGCGACAGCCTGGCA11.0309278350515463No Hit
AGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCAA11.0309278350515463No Hit
CTGTTTACACTGGCTCTGTTATTCTTCTAGTCTTTCAAACTCCAACCTCA11.0309278350515463No Hit
CACTAGAAGCACCACCACGTGGGGACCTTCGGGGAGCCGGAACGTCAGGG11.0309278350515463No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT11.0309278350515463No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA11.0309278350515463No Hit
TATATACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGG11.0309278350515463No Hit
GTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCCTCT11.0309278350515463No Hit
GTTGGATTCTGTCTACGGATGTCCCCAATCCTTACTCCTCCAATCTGTGT11.0309278350515463No Hit
GACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAGACA11.0309278350515463No Hit
ATGTAGATGGATTCGAGCCGAACGGCTGCATTGAGGGCAAGCTTTCCCAA11.0309278350515463No Hit
AAAGAGAAGAAAGAAGAGCTCCAGGATTCTAAAATTGCTCCATTGATGGA11.0309278350515463No Hit
AAGCTGATGTTATTAATATCAGAAAGAAATAGACTTTAAGCAAAAGGCAT11.0309278350515463No Hit
GCTTAATGCTGGGCCATATCTCTTGTCGCCATCATCCACTTCATTCTGAG11.0309278350515463No Hit
GCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTGGTGGGTTCACTTT11.0309278350515463No Hit
GGGGATGTGTTTGTCATAAGGGAACCATTCATATCATGCTCCCCCTTGGA11.0309278350515463No Hit
AATGCAAAACACCTTGTAAAACACACAAGGGTGCTAGAAACACCACCCTC11.0309278350515463No Hit
CCTGGACCCTGTTTGAGATGCTTCCCGCTCTAATCGGTAACTATATTAAT11.0309278350515463No Hit
ACCAGGGCTAGAAGCGGAACCACAGTCAGGACTGGAAGTAAGACGTGAAC11.0309278350515463No Hit
TTGTAAAATTGCTCCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGG11.0309278350515463No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11.0309278350515463No Hit
GGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAAT11.0309278350515463No Hit
GTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCTGGGTCCAAA11.0309278350515463No Hit
TGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTAAGCG11.0309278350515463No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT11.0309278350515463No Hit
TTCCCCTCCTTTCTCATATAAACTACCCTTTGGGCTCCCTGCTCTGCCTC11.0309278350515463No Hit
GTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTT11.0309278350515463No Hit
GGACAGTTTGGATCGCAAATAGTAGCTTTGCCCCAACTCCTTCAGTGCAT11.0309278350515463No Hit
GGAAAAATACTGTGTTCTTGAAATAGGAGACATGCTCGTGAGGACTGCGA11.0309278350515463No Hit
TGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAATGGACATGATT11.0309278350515463No Hit
GAGGCAGTAGGCAGGGGTTTTAGAGAGGCAGAGCAGGGAGCCCAAAGGGT11.0309278350515463No Hit
CTTCAATTTCTTTCATCAGTGTTTTCTAGTTTTCAGTGTATAGATTTTTT11.0309278350515463No Hit
ATTTTCAGTGTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGAC11.0309278350515463No Hit
GCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCCCCAGCCTTGAAA11.0309278350515463No Hit
CATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTT11.0309278350515463No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTTCCAACACTACGTCCCCTT11.0309278350515463No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA11.0309278350515463No Hit
CATCCAAGCAGGAGTGTAAAGCTTATACAGTCCAGGCAATTTAGGGGTCA11.0309278350515463No Hit
GGGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCCGCCATGGATGA11.0309278350515463No Hit
GCAATAAGCAAAACCCAGGGATAATTAATAAGGCACTCCTCGATTGCTTC11.0309278350515463No Hit
CTCTGAAGGTCCTGGGTGGGGCTGGAGAGTCTGCATTTCTCACAGGCTTG11.0309278350515463No Hit
TTTCATTTCTGCGCGGGAGGGAGTGACGGTCTCCCATTCTCATCACCGTT11.0309278350515463No Hit
CCGATTGATGGACCACTACCTGAGGATAATGAACCAAGTGGGTATGCACA11.0309278350515463No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG11.0309278350515463No Hit
TGAAAAAGGAGCAGTCAGACATCAGGAAAGACTACTTTGGGGAGAAAGAA11.0309278350515463No Hit
CAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAAT11.0309278350515463No Hit
CACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA11.0309278350515463No Hit
GACTTTGCCCCCTGTGGGGCTGTTGGCCCTAATCAAAACCTACGCCGCTG11.0309278350515463No Hit
ACGGCTCCCCCCCGGCGCCCCAGTACTTCCTTCCCTTGCCGTGCTGAAGA11.0309278350515463No Hit
CATTTGACACTGTCCAAGTAATAAAACTTCTCCCCTTTGCTGCTGCCCCA11.0309278350515463No Hit
GGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCC11.0309278350515463No Hit
GGGAGAAACCTTGTTTTACGGACCAATTCTCTTTCTAGCATGTACGCCAC11.0309278350515463No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC11.0309278350515463No Hit
GGACAGTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTC11.0309278350515463No Hit
AAATAAGACAGGGACATTTCAATTCACAAGCATTTTTTATAGCTATCGAT11.0309278350515463No Hit
TCTCACTTCTCCTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGG11.0309278350515463No Hit
CACATGCAGTCTATAGCCATTCACCCCCACATTGTGCGAAACAAAAGATG11.0309278350515463No Hit
GGCCAGGGCCCGCCCCGGCCGCTGTCCCGCAGCCCGCGGCTGCCCGTTCT11.0309278350515463No Hit
AAAGAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAAT11.0309278350515463No Hit
GACTGGAAAGTGTCTTTGCAGGAAAGAACACAGATCTTGAGGCTCTCATG11.0309278350515463No Hit
CTTCGTGTGTGGAGATCGGAACCCAGCAAATTGACTCCCCACGGTCCAGT11.0309278350515463No Hit
CAGTGGGCCTCACTCCTGACACCTAGTTCCCAAACTGGCTCTTTCCACCA11.0309278350515463No Hit
TCAGTATCATTTCTCAAATGAGACCTTCCTTTTATAATGAACCCATACAG11.0309278350515463No Hit
GGATAGGGGAGGTTATCTATTAATACCTGACACATAAAAAACCAAGCCCC11.0309278350515463No Hit
GCCCCGGCAGGACCTGGACCCGGTCATGGACCTGTTAGCGCTGTATCAGG11.0309278350515463No Hit
GTGTCTGGCCATTGCCCGGGCCCTTGTGCGGCCCCCACGAGTCCTCAGCC11.0309278350515463No Hit
GGTTGAAACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAA11.0309278350515463No Hit
GGACCCAGCTTGCTAGAGATCTGGGCTCTGGCTCATCACTGTCATACTGT11.0309278350515463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAAAA50.0145.03
ACACCTT50.0145.09
GCAAAAC50.0145.04
ATGCAAA50.0145.02
AATGCAA50.0145.01
CGTCTAT50.0145.0145
AACACCT50.0145.08
CAAAACA50.0145.05
AAACACC100.072.57
AAAACAC100.072.56
Produced by FastQC (version 0.11.5)