FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCCAATGATAAGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAA12.380952380952381No Hit
TCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGCCGTTGCAGGAC12.380952380952381No Hit
TATATGTAATCATTGCCAGGAACATTCGAGGATTTTGATTTTCATTACAC12.380952380952381No Hit
CTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCT12.380952380952381No Hit
GCGTGGACAGATTCTACAGGACCTGCAAGTTTGTGGGAATAAGCATGAGC12.380952380952381No Hit
GTTGTAATTGAATACTGGAGAATTGCATCTTACCAGTATCCTAAACCCTG12.380952380952381No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG12.380952380952381No Hit
GTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCCCTCCTTCCGTAGCC12.380952380952381No Hit
GTATGGAGTTCTCACTCACTGACACGAGACTGGAGCCACACAAATGGGAA12.380952380952381No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGAAAATGGAG12.380952380952381No Hit
GGGACTGGAGCTGCCCTTGCGGCTATTCTGGGTGGACAGCACAGAGTGAT12.380952380952381No Hit
GATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACATGACCAAGGAATTCT12.380952380952381No Hit
TTACAGGATATATGAGCGAGAGAGAAGACCAAAGACTCCCACAGACATTA12.380952380952381No Hit
GCATTAAGAAAACCCCAGGTATCATTAATAAGTCACTCCTCTATTGCTTC12.380952380952381No Hit
GTAGTACAGAGCAACTGTGAGGATGGAGTTGAACCAAGAAGCATTAAGCA12.380952380952381No Hit
GTGTATAAGATGGGGAACCGAATTCACTAGTTCCGCCACTAGGTGAGAGA12.380952380952381No Hit
GATGAAGGCACATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTATCAT12.380952380952381No Hit
GCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGCAAGGCCGTATTT12.380952380952381No Hit
CCTATATGAACTGCTACGGAAAAATTTGTCCAATAGATTGCAGCATGGTG12.380952380952381No Hit
AAAGCCAACAGTGTAAGAATCGGTTCCAAGGTGGATGTGTTTGTCATAAG12.380952380952381No Hit
GGGCCATATCTCTTGTCTTCGTTGCCCAAAATGAGAAATCCTCTCAGGCC12.380952380952381No Hit
GGTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCATGGGTGACT12.380952380952381No Hit
GTCCCAAACGAAAGAAGACAATATATCTAACTTCCAAACACCAAATCTCT12.380952380952381No Hit
AGGCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAG12.380952380952381No Hit
GGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAATGTTATACAAC12.380952380952381No Hit
GTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAACCCCAGGGATCATT12.380952380952381No Hit
GTGCTGGTCTGAAAGATGACCTTCTTGAATATTTGCAGGCCTACCAGAAG12.380952380952381No Hit
CTTGTGAGCTGGGTTTTCATTTTGTCTCTTCAGGATGACCGCTTGGCTGT12.380952380952381No Hit
CCCATAGACTCCTACTGGCCATTTCTTGTCTTATAGTGAAAAGCCTAGTT12.380952380952381No Hit
GTACAGTGGATTCGTAAGGACACTTTTCCAGCAAAAGCGGGATGTGCTTG12.380952380952381No Hit
CTGAAAATAAGAGTACATGAAGGGTATGAAGACTTCACAATGGGTTGGAG12.380952380952381No Hit
AATACAAACAAGCCATTTTTTCATTAAGGACAATTTAAATACATTATTAT12.380952380952381No Hit
TCTTTTGACATTAGGGAAAGCTTGCCCTCAATGCAGCCGTTCGGCTCGAA12.380952380952381No Hit
GGTCCATCTGTTCATCCTCAAGAATTCCCCGTTGGCTTGTGTTGAGAATA12.380952380952381No Hit
GCTTAGTGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC12.380952380952381No Hit
CTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGACCAGGA12.380952380952381No Hit
GTGTTTATTTTGATGTGTTGCAGTGAGCGGTATGGACGTGGGGGGAGGAG12.380952380952381No Hit
CATGACAAACGCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCT12.380952380952381No Hit
GACGGGAAAGTGTCTTTGCAGGAAAGAACACAGATGTTGAGGCTCTCATG12.380952380952381No Hit
ATCCCGCCCAGTGAGTACCCTTCCCTTTCAAAGTCCTGCCCACTTGCTAA12.380952380952381No Hit
CATGTGATTTCACTAGAATAAGGATAACAGGAGCTTTCCTCATAGTGATA12.380952380952381No Hit
TGGGAAAACCACTTCCATAATCCCATCCTGTGCCTCCTTGGCACTGAGCT12.380952380952381No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)