FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879979
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA125511.426284036323594No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG81770.9292267201830953No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT81280.9236584054846764No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA70810.8046782934592758No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT69890.7942234985153054No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA60650.6892209927736912No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT58940.6697887108669639No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC51150.5812638710696505No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG50570.5746728046919302No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC49470.562172506389357No Hit
CCATACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGA48530.5514904332944308No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC44320.5036483825182192No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG44260.5029665480653516No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG44190.502171074537006No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG41570.47239763676178637No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG41250.46876118634649233No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG38590.438533192269361No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC37960.4313739305142509No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG36640.41637357255116314No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC36300.41250984398491325No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG36110.4103507015508325No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT34000.3863728566249876No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT33590.3817136545303922No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG32710.3717134158883337No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT31310.3558039453214224No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG30590.34762193188701096No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG28440.3231895306592544No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA28250.32103038822517355No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA27040.30728006009234315No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG27000.30682550379043133No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG26090.296484347921939No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG25850.29375701011046856No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT24880.2827340197891086No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC24040.27318833744896187No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG23520.2672791055241091No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC23160.26318809880690336No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG23120.2627335425049916No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA21070.2394375320320144No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC20570.2337555782581175No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA20170.22921001523900003No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA20120.22864181986161033No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT20100.22841454171065445No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT18250.20739131274723602No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA18210.20693675644532428No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT18060.2052321703131552No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT17710.2012548026714274No Hit
TCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACT17430.19807290855804513No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC17430.19807290855804513No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA17430.19807290855804513No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC17180.1952319316710967No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC16990.19307278923701587No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA16900.19205003755771444No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA16870.19170912033128062No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT16740.19023181235006745No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA16710.18989089512363364No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA16710.18989089512363364No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG16580.18841358714242043No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC16180.18386802412330294No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA15850.180117934632531No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG15390.17489053716054587No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC14550.16534485482039912No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA14550.16534485482039912No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA14530.16511757666944324No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG14500.16477665944300943No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA14300.16250387793345067No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA14300.16250387793345067No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG13990.15898106659363462No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC13920.15818559306528907No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG13880.1577310367633773No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT13690.1555718943292965No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA13690.1555718943292965No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT13670.1553446161783406No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA13580.1543218644990392No Hit
CCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTG13150.14943538425348785No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG13060.14841263257418644No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13040.14818535442323055No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT12940.14704896366845116No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC12790.14534437753628213No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC12430.14125337081907638No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC12380.14068517544168668No Hit
GGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGA12290.13966242376238525No Hit
ATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATGCA12240.13909422838499555No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG11980.13613961242256917No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA11950.13579869519613536No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG11890.13511686074326773No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA11860.13477594351683392No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA11810.13420774813944425No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA11780.1338668309130104No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT11630.13216224478084135No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG11550.13125313217701787No Hit
GTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCCCAATAGAGC11510.1307985758751061No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT11460.13023038049771643No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC11300.1284121552900694No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA11270.1280712380636356No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAC11210.12738940361076798No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG11170.12693484730885624No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT11160.12682120823337828No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT11020.12523026117668717No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG10900.12386659227095191No Hit
CACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGT10830.12307111874260636No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT10800.12273020151617253No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA10780.12250292336521668No Hit
TAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAA10710.12170744983687112No Hit
ATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG10650.12102561538400347No Hit
CCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAA10640.12091197630852554No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT10600.12045742000661379No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC10570.12011650278017999No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG10490.11920739017635647No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG10490.11920739017635647No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT10450.11875283387444474No Hit
CGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTT10420.11841191664801091No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG10400.11818463849705504No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC10320.11727552589323154No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10240.11636641328940805No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA10110.11488910530819485No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT9960.11318451917602579No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT9840.11182085027029054No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA9660.10977534691168765No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA9620.10932079060977593No Hit
ACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAG9620.10932079060977593No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC9510.10807076077951862No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA9230.10488886666613635No Hit
ATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAA9220.10477522759065841No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA9090.10329791960944522No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG9090.10329791960944522No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT9020.10250244608109967No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG9000.1022751679301438No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA8910.10125241625084234No Hit
TCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTT8810.10011602549606297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACG151.2298537E-4145.00302145
AGTAGAA14600.0117.220861
GCAGGTA6950.0116.8502352
AGCGAGC203.8655815E-4108.75226145
CAGGTAC7650.0105.210223
GGTACTG7750.0101.981455
GGTCAAA9300.095.910891
AGCGAAA2400.087.62591
AAATATA10300.086.589315
GTCAAAT10750.083.639152
ACTGATC9700.082.213538
GTACTGA10000.079.035636
CGAAAGC2650.076.614073
GTAGAAA22750.075.21892
GCGAAAG2700.075.195292
AGGTACT11450.072.826384
GATCTTA1300.072.517991
ATACCGG406.6545574E-572.517991
TTCTGCG406.662057E-572.50151145
AAGTGCG300.001935505272.50151145
Produced by FastQC (version 0.11.5)