FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT80.3686635944700461No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT60.2764976958525346No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT50.2304147465437788No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT50.2304147465437788No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC50.2304147465437788No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT50.2304147465437788No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA40.18433179723502305No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT40.18433179723502305No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC40.18433179723502305No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA40.18433179723502305No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG40.18433179723502305No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG40.18433179723502305No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC40.18433179723502305No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG30.1382488479262673No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT30.1382488479262673No Hit
GGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATTGCATGATCAAGG30.1382488479262673No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA30.1382488479262673No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG30.1382488479262673No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC30.1382488479262673No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30.1382488479262673No Hit
GCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAG30.1382488479262673No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT30.1382488479262673No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG30.1382488479262673No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT30.1382488479262673No Hit
GTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGATGAGTATTGGA30.1382488479262673No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT30.1382488479262673No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT30.1382488479262673No Hit
CTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCAG30.1382488479262673No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA30.1382488479262673No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA30.1382488479262673No Hit
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG30.1382488479262673No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT30.1382488479262673No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA30.1382488479262673No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT30.1382488479262673No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.1382488479262673No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT30.1382488479262673No Hit
CATGAAGACATTCTTTGGCTGGAAAGAGCCTAACATAGTCAAACCACATG30.1382488479262673No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT30.1382488479262673No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)