FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_C01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_C01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1666119
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG32280.19374366416804562No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT26020.1561713178950603No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA23430.14062620977253126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4400.052.7388841
CAGCTCG13800.044.6549079
TATACTT12800.037.38885
TATATAC32200.035.1298523
TATACAG22450.031.6531775
ACCGGTA1153.1600273E-431.526792
TATAACG1153.1600273E-431.526794
GCGAAAG7500.030.9382862
CGAAAGC7600.030.5312083
CTACGCC3453.6379788E-1227.3232173
CATATAT17550.027.2709621
CATACAT13050.027.2216828
ATATACT18550.026.9719754
TACTTAC18300.026.9393567
GTATATA25650.026.8577651
TTATACT6900.026.2723244
TACAGCT23600.026.1117697
ACAGCTC24650.025.2936158
ATATATA21050.025.1465472
TGTATAT25100.025.1350171
Produced by FastQC (version 0.11.5)