FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_B12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_B12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTACGA12.4390243902439024No Hit
ACAATGACCTTGGACCTGCAACGGCCCAGATGGCTCTTCAATTGTTCATC12.4390243902439024No Hit
CACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGA12.4390243902439024No Hit
ATATGAAGCAATCCAGGAGTCCCTGATTAATGATCCCTGGGTTTTGCTTA12.4390243902439024No Hit
ATTTGAGTTAAAGAAGCTGTGGGATCAAACCCAAGCAAAGGTAGGGCTAT12.4390243902439024No Hit
ACTGGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACACAGAGA12.4390243902439024No Hit
CCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAA12.4390243902439024No Hit
GATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAA12.4390243902439024No Hit
GGTGACCCGCATGCACTATTGCAGCACCGATTTGAGATAATTGATGGAAG12.4390243902439024No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTACATTCCCTTATA12.4390243902439024No Hit
GGTGGTGAGTTGGGATGGGGTTTGCGCAAATGAGGGGGGGGCGCGAATAG12.4390243902439024No Hit
CCCTCCGTGTTCCTCCAATGTAACGGACCCGGTTATCGGTGGAATCAGAC12.4390243902439024No Hit
GGCCTCAGGCACTCCTTACGTAGAAGGCCCTCTTTTCAATCCGTATTTAA12.4390243902439024No Hit
GCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAA12.4390243902439024No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCCATCC12.4390243902439024No Hit
AGCTCAAATTTTTCTTCAATTGTATCTTCTACTATTGAGGACTGACGAAA12.4390243902439024No Hit
ATCAAGTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATT12.4390243902439024No Hit
GCCTCCAACACCAGGTCAAGCTTTCAGGTAGACTGGTTCCTTTGGCATAT12.4390243902439024No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC12.4390243902439024No Hit
ACCTCCGTCAGTCCATGTTTGAGTAATGAGTTGAACAAAGATGAATTAAG12.4390243902439024No Hit
CCATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCT12.4390243902439024No Hit
GTCCCCTCCTGCTTGTATTCCCTCATGGTTTGGTGCATTCACTATGAGAG12.4390243902439024No Hit
GCCACACCCTGGCCATGCCGATCTCAGTGCCAAGGAGGCACCGGAGGTGA12.4390243902439024No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG12.4390243902439024No Hit
GGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATG12.4390243902439024No Hit
GGTTGGGTTCAAAGCGACCCGGTTCATTGAAAGTGGAGTAACACGGAGGG12.4390243902439024No Hit
GGCCACTGTGGACCATATGGCCATAATCAACATGTACCCAGCAGGACGGC12.4390243902439024No Hit
GATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAG12.4390243902439024No Hit
GTTTCGGACTGACGAAAGGAATCCCATAGACTCCTACTGGCCCTTTCTTG12.4390243902439024No Hit
TTACAAAGTCTTCCCCGATAATCATCATCCATTAGCTCCCATTTTAAGCA12.4390243902439024No Hit
CCAAGTCTGAAAGCCTCTTATAGGCGCGCTGTCTGTCCATTAACTTCAAT12.4390243902439024No Hit
ATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGGTAC12.4390243902439024No Hit
CAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCT12.4390243902439024No Hit
GAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGC12.4390243902439024No Hit
GGCACACCATTTGAATGGATGTCAATCCGCCTCTACTTTTCCTAAACATC12.4390243902439024No Hit
ATGTTAACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCA12.4390243902439024No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACCCTAATTGATGGCCATCC12.4390243902439024No Hit
TTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATT12.4390243902439024No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC12.4390243902439024No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCGAGACTTAATT12.4390243902439024No Hit
CCAATACTCAGAAAAGGGAAAGTGGACTACATACACAGAGACTGGTGCAC12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)