FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_B11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_B11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATACATATTATTAGGGCAGAGTCAACAGTTTTAAGTGAGCGCTCT150.2827521206409048No Hit
TCCCTGGACAGGCCGGCTGGGACATCACAGCAGGTGCTACAGCCTCCCGA130.24505183788878418No Hit
GTATTAACTCTGAATAATAACGAGTCTACGTGGATGCCCCATCCTACCAA110.20735155513666353No Hit
CCCCAATAGTTGACGTTTCTATAACTTTAATTCCTCGGGCTTTTGGCCTT110.20735155513666353No Hit
GATCTATATTAGAGAAGATCTAAATAATTGGAAAAAACATCCTGTGTTCA70.13195098963242224No Hit
ATTCTCTTCTCATCCACTCTTAGGACTTTAAATACTACCTGTATTGTAAC70.13195098963242224No Hit
CCCTTGAATCCCTCTCTGGGAGAGAAGAGGTTTCTGTTGTTCGAAGCCAC70.13195098963242224No Hit
CCATATGGATGAAGCAGACATCCTGGGGGACAGGATTGCCATCATTTCCC70.13195098963242224No Hit
GTATAAAGTAGGTAAACCTCTAGCTTTAATCTTTTGCCATCTCTGATCTC70.13195098963242224No Hit
GTAGGAATATAGGTGTTTACTATATTCTCTTAAATTTCCTGTTGGACTGA60.11310084825636192No Hit
ACCTGACCCTGGTCAAGAAGGATGTGGAATCCTCCCTCAGTTCCTGCAGA60.11310084825636192No Hit
CTCTTACTTGGGCTGAAAGCCAGCTGGCAGAGGGGAGCCACGACGCTCTG60.11310084825636192No Hit
GTGTAGGGTGACCTGACTCCATCCCCACTCCAGAACTTGGCCTCAGCACC60.11310084825636192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTACT100.0068944762145.02
Produced by FastQC (version 0.11.5)