FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_B07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_B07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4818
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT110.228310502283105No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA100.20755500207555005No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT100.20755500207555005No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG80.16604400166044No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC80.16604400166044No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG70.14528850145288502No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC70.14528850145288502No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC70.14528850145288502No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG70.14528850145288502No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.12453300124533001No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG60.12453300124533001No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA60.12453300124533001No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC60.12453300124533001No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG60.12453300124533001No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG60.12453300124533001No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT60.12453300124533001No Hit
TGATTACATATATCACCAGAAATCAACCCGAGTGGTTCAGAAACATCCTG50.10377750103777503No Hit
GGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGAGAATTCAT50.10377750103777503No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA50.10377750103777503No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA50.10377750103777503No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA50.10377750103777503No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG50.10377750103777503No Hit
GGATAAGAGTCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA50.10377750103777503No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA50.10377750103777503No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT50.10377750103777503No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG50.10377750103777503No Hit
GACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACAC50.10377750103777503No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG50.10377750103777503No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC50.10377750103777503No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT50.10377750103777503No Hit
GATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAA50.10377750103777503No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG50.10377750103777503No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA50.10377750103777503No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.10377750103777503No Hit
ATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCCACAAT50.10377750103777503No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT50.10377750103777503No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG50.10377750103777503No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT50.10377750103777503No Hit
CATTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC50.10377750103777503No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.10377750103777503No Hit
GGTAAGAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGAC50.10377750103777503No Hit
CCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTTGAGTTAAAGA50.10377750103777503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATG100.006874048145.03
ATATAAA100.006874048145.01
Produced by FastQC (version 0.11.5)