FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG24.651162790697675No Hit
GTGCTAGTTTGGTCAACGTTTTAAAAGAGAGAGGAGTAAAATCATAGGGA12.3255813953488373No Hit
GGCTAAGGCTGTCCAAAATCCGTCAACATCAAATCTCGTGGAGGGTGACA12.3255813953488373No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG12.3255813953488373No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG12.3255813953488373No Hit
CTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCT12.3255813953488373No Hit
GCCCCAGTGTCGGGGCCAGAACTTCCACTTGTTAGCCACTTGAGGCCATC12.3255813953488373No Hit
CAATCATGCTCTCAATCTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATT12.3255813953488373No Hit
GAAGAGCAACAGCTATTCTCAGAAAGGCAACCAGGAGATTGATCCAGTTG12.3255813953488373No Hit
CTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCATCT12.3255813953488373No Hit
GTCGCATTGCAACAACTACTTAAGTGCATTTGTGAGGAAGGAGTTGAACC12.3255813953488373No Hit
CAAGGGTAGATAAACTAAATCAAGGTAGCCAGACTTATGATTGGACATTA12.3255813953488373No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT12.3255813953488373No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG12.3255813953488373No Hit
CAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCA12.3255813953488373No Hit
GGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTACACAAAACCG12.3255813953488373No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCGCCATAGCCTC12.3255813953488373No Hit
CTAATGTCGCATTCCCGCACTCGCGAGATACTCACTAAGCCCACTGTGGA12.3255813953488373No Hit
TGGTGAAGGAGTGTTCTTCTTGCCAGCTGGGAGGGAGGAGCAGTGGAGAG12.3255813953488373No Hit
GTGGAGCAGGAGCCTGTGCTCTTCTCGGGTTCTGTGAGGGACAACATTGC12.3255813953488373No Hit
CAGTTAATATATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC12.3255813953488373No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12.3255813953488373No Hit
GCCGTGATCTCCCACATCAGGGATTCCTCATCAGGTATTTTCAACTTCTC12.3255813953488373No Hit
GCTAACCACCTTGGCGATGGCAGCAGGACCCCCGTAAAGACACGGAGACA12.3255813953488373No Hit
CCCCGGCCAGTCCAGTCCGCCGATGAGGACCCCACCGGCAGTTTTGAAAG12.3255813953488373No Hit
AGAACCAACAGTTCGGCATTGTAAGTCAAAATGTCAAGGAAACCATAATC12.3255813953488373No Hit
TCTCGTTTGTTGTCAAATGAAGGTTAGGCCTTTGGCGTTATGTGTAAGGT12.3255813953488373No Hit
GAACAGTATAGAAGACTTAACATCTAATGTCCTATTGCGAAACTGTCCAG12.3255813953488373No Hit
GTAGGTACAGATGCATTTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT12.3255813953488373No Hit
ATACGGAGTCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTACTGGA12.3255813953488373No Hit
CACTAAGGAGAGAATAAAAGAACTGAGAAATATAATGTCGCATTCACGCA12.3255813953488373No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTAGCTCCAATATG12.3255813953488373No Hit
CCGTATAGTGTATAAGTGTGGTCCTCCATCTGATCCTAAGATCCCTACCG12.3255813953488373No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG12.3255813953488373No Hit
GCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATT12.3255813953488373No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTATCTGTCTGGCT12.3255813953488373No Hit
ATAAACAACAGCCCAAAAAAACTCTTTATTCTTATTTCAACAAAAAAAAA12.3255813953488373No Hit
GCTCATTCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACA12.3255813953488373No Hit
ATGCTCATGATCTCAACAGAGGAATGCTCTTTAAAAAAAAAAAAAGGCAA12.3255813953488373No Hit
GCATGTGTCAGCATGGAGTTCAACCAAGATGCAATAAGCAAAACCCATGG12.3255813953488373No Hit
CGCATATATACTGTTGAATACAGATTTTGCCAGAAAGGTCCTGCCCACTT12.3255813953488373No Hit
GCCCATGCACGTGACCCGCGGACCCTCGCGTTCTCTGCCCTCTCCACCCG12.3255813953488373No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)