FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_B03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_B03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCCTTCCGACTCCGTCTGGCAATCAGCAGCCTCTTCCACAGAAGGGC12.272727272727273No Hit
GCATAGTCTGAGCAGCCAGCAGGGCAAAATGAGGGTGGGGGTAAGCCAAG12.272727272727273No Hit
AATATATTCAATATGGAGAGAATAAAAGCACTGAGAGATCTAATGTCGCA12.272727272727273No Hit
CTCTTACTTGGGCTGAAAGCCAGCTGGCAGAGGGGAGCCACGACGCTCTG12.272727272727273No Hit
GTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACGACG12.272727272727273No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTTTTTGGAGGTTGCCCGTT12.272727272727273No Hit
GTTAAAAAAAATTCCAGTACCGCCCCTCAGCTGAATACCAACAAGACATT12.272727272727273No Hit
GTATAAAAAAATGAAGCCAGTAAAACCAGGAAAGCACTAAAGTAAAATTG12.272727272727273No Hit
CCATTACCAACTACAAATTCATTACGAATTCTAAGTAAGTCTCTCATGCT12.272727272727273No Hit
CAACATGGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAA12.272727272727273No Hit
CATTTGAAGCATCATGCGTGGAGAGGTGAAGAAGCCGGCCCAGACCGATG12.272727272727273No Hit
GGTTCACTCCGCAGACCTATTCTCTTCTCATCCACTCTTAGGACTTTAAA12.272727272727273No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGAGTGATGATCC12.272727272727273No Hit
ACTGATAACATTCCTACAATAAAGGAAAACACAAAAAATACCTAGCGTCA12.272727272727273No Hit
CAGTTCCTGCAGAAACAGCAGTAGCACTGTGTCATACCTGAAAAAGGAGG12.272727272727273No Hit
AGGTAGGGCACCCCAGGCCAAGGAAACTGCAAGAGCCAAAGCCCTGGGGA12.272727272727273No Hit
CACTCCAACAACTTGCCAACGGTCTAATAAAAAATTTGCTAATCAATTAG12.272727272727273No Hit
ATGTCTTCCCAACCGTTGGCAGGAAAGAAGAGCAGAGCATTTTCTGCTGT12.272727272727273No Hit
TCTCTCCTCTGGTCAGTGGGGTCCTAGTTAAAGCCTTTCACTCAAACTGC12.272727272727273No Hit
GTTGTATTATTTGGACAGTGTCAAATTTCCCAAGAAAATCCCGCATTTGC12.272727272727273No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA12.272727272727273No Hit
AAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTTTTTTGC12.272727272727273No Hit
GAGCGTGAGGTGTGACTCCGGCTTCCCATACCCTTGGAATGTCTGAGTTA12.272727272727273No Hit
GGGCAATGATGGAACAAGTAAGAGAAAGTCGAACCCCAGGAAACGCTGAG12.272727272727273No Hit
GTATATATGAAACTAATATCACACACAATGTTAATTAAACTGGAATTAAA12.272727272727273No Hit
ACTCCAGAGGTTTTTATAAATAACAAGCATTCGTGTTAATATCCTACGAA12.272727272727273No Hit
CATTAATGACTTTCCCTTCTATCTGTTCCAAGAAACACCAAGAAATGGAG12.272727272727273No Hit
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT12.272727272727273No Hit
GCTCTGAGCTTAATCCTCTCCTGAGCTTGCCTACATGTCTGAGGCCCTCA12.272727272727273No Hit
CAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCG12.272727272727273No Hit
GGTATGAGGTCACTAGAAGAGAATACCGGTTGCCCCTTTATGCTCATAGC12.272727272727273No Hit
ATACTAGGCCATAAGGACCAGGAGTGGAGCAAATACCAATCAACAAAAGG12.272727272727273No Hit
ATCATATACGAACTCAGGACAGCCACCTGAGAGAAAGCTCGGCCGTGGGG12.272727272727273No Hit
CTTTAGACAATGCTAGGATTTGAGCCCAGTTCTGTCTCGTTATAGAAGCG12.272727272727273No Hit
CATACACACAGTGATCATCAAACACTTAGAAGTGTCAGAAGAGAGGCTGA12.272727272727273No Hit
ATGCTGCTGATCCTAATGATTCTGATGTAGACCCAGAATCCAGAGAAACA12.272727272727273No Hit
TTGCGTATCCCAACCGCCCAGACCAACCTGTGCTCAAGGGGCTGACGTTC12.272727272727273No Hit
CATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGGGCATACAATAATG12.272727272727273No Hit
CATCCGCCACCCACGCACACCAAAAGCAGACACAGAATGTCTGGATTACC12.272727272727273No Hit
ATGCGCAAGTGTTTTACCTTCATCCTAACCCCTAAGCCCCACCTAAAGAA12.272727272727273No Hit
GCTCTTGATGTGATCTGAAGGCTGATGTCTGTTGAACTGGGCCCACAAGG12.272727272727273No Hit
GGTCTTAAATCAGAACCCGAGGGGCCAGGATGGCTCACGGTGGTGTCCGC12.272727272727273No Hit
GCCCCACCCGCCGGGCCTGGGCTCTTGGGACAGGCCTGCAGTGAGCTCTC12.272727272727273No Hit
AATATATTCAATATGGAGAGAATTAAAGAACTGAGAGATCTAATGTCGCA12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)