FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGAGGACTCCGGGGACGGAGAGGGCAGGGCTTGCTTGATCGCGTGGCG14.0No Hit
GTGCTGGAGTATTCATCTACTCCCATTTTGCTGACTCTTATCCCTCTCAG14.0No Hit
AGTTTGGCCTTCTTTTCATTGCCCCCTACTGGGAGCCCAGACTGTTCAAG14.0No Hit
CTGGTGCCATTTGGGACAGCTTGCCCTCAATGCAGCCGGGCGGCGCGACT14.0No Hit
TACTAGGGAAAGGGTAAAGTTTAGATAGTAAAATAATGCAGAGTATCACA14.0No Hit
TTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAG14.0No Hit
CATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATG14.0No Hit
CCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCT14.0No Hit
GAATTAGTCATCATCTTTCTCACAACATTTGCCAGTTTGGCCTTCTTTTC14.0No Hit
GGATAGCGCAGTTAAACTATACAAGAAGCTCACAAGAGAAATAACGTTCC14.0No Hit
GCATCATATTCCATGCTATTGGCTGGACCATGGTCTGGTATTACCACAGC14.0No Hit
TTTGCATGGTGATCACTATCTCTTTGACACAATACACCCCCTGCATCTAG14.0No Hit
CTATTAGAAGCGGGCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG14.0No Hit
CTTATAACTTCTGTTCGCATGTCGGATGTCCGTCCTTCATTGTTCCCGCT14.0No Hit
GTAGAAACAAGCTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT14.0No Hit
AGGTTCGAGAGTAAAAGAATGAATATTCGAACACAAATACCCTCATAAAT14.0No Hit
ATTCACAATGGGTGGGAGAAGAGCACCATCGATTCGCAGAAAGGCAACCA14.0No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA14.0No Hit
GGAGTTTTTTCTTCAAAATTCATGTCTATGCTAATTCGCTAGACACCTGC14.0No Hit
CTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAGGT14.0No Hit
GTGGATCTGAAATGATAATACCCGATCCAGCAGTTATTGCCATTGCGAAT14.0No Hit
GTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATTG14.0No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT14.0No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC14.0No Hit
GTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCT14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)