FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208072
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT17900.14816997662390985No Hit
GTACTGAAGAACACAGTAAGTCAGGATGGGACAAAGGCAAGGCTGGAAGG15300.1266480805779788No Hit
GTCCTAGCGAATGGCCCTGGCACTCGCAGGCTTGTGGAAACGGCTAAGCT14160.11721155692707057No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA13410.11100331768305201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTT451.2316177E-680.571225
AGCGAAA7050.062.745291
CGAAAGC7450.059.373963
ATCGTTG701.6942473E-551.7957846
ATATCGT701.6942473E-551.7957844
ACATCGA1405.456968E-1251.7829178
GCGAAAG8550.051.7352032
TATAAGC1851.8189894E-1243.116493
ATAAGCG1201.9747313E-742.2893878
CATCGAG1557.403287E-1042.0945059
TAATGGT3600.040.285614
ATACTTA3100.039.7657976
TATCTAG1209.552758E-636.2570465
GCGATTA800.002035666536.254047145
ACAGTCG1604.59313E-836.2480438
AACCGGT3300.035.158356
ATAGACC9550.033.4096373
GATCGTA900.003624339832.229821
GGTGTAT900.003629486832.2204868
ACTGTGC8250.031.6346558
Produced by FastQC (version 0.11.5)