FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATAGAATTTTTTTTAATCTTAAAAAATATCAAAATAGAATACTAAAA13.0303030303030303No Hit
GGTCTCCTATATGAACTGCTAGGGAACAATTTCTCGAATAGAGTGCCGCA13.0303030303030303No Hit
GTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGT13.0303030303030303No Hit
GTATAATAACCACCATTAAAAAAAAATTTTTTTAAACAGCCTCCAATTTA13.0303030303030303No Hit
GGGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGC13.0303030303030303No Hit
GATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGACAACATGACCA13.0303030303030303No Hit
CTACTAGATGGACCAGCATAACGGAGTACGGGGATGAGGCTGGGCAGGGT13.0303030303030303No Hit
ATTACGGGGTAAGTGACATCGTACGTGGAAGAGTAAGGTACAAACAGGTT13.0303030303030303No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTCTCCGATTGGC13.0303030303030303No Hit
GACATATGTTAACATCAGCAACACCAACTATGCTGCTGTAAAGTCAGTTG13.0303030303030303No Hit
GTGAATCTCTTAAATAGGACTGCACATAGTCCTGTGATACAACCATTCCT13.0303030303030303No Hit
GACTAACATTGAAAAACAACAAAATGATAAACAATTAACTTGCACCAGAA13.0303030303030303No Hit
GTTGAATAGATCGCCAAAATCTGGTAAATCCTTGTTGATTCCAGCTTTAC13.0303030303030303No Hit
ATGGAGAAAGTACCATTTGCAAAAAAAAAAAAAAAAAAACCAAATGAGGT13.0303030303030303No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA13.0303030303030303No Hit
GTTGAGAAAAGCTATATTGCCAAAGTTTCCACGACCAAAATGGCAAAAAC13.0303030303030303No Hit
CTGATGATCCGCTTGTCCTTTTGAAAGTGAACCCACCAAAACTGAAAGAT13.0303030303030303No Hit
AAGCAGGAGGGGTTAGCCCCGAGATGGGTATTAAGGAGGGCACGAATTGC13.0303030303030303No Hit
CATATACTCTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCCTTCAAA13.0303030303030303No Hit
CTGTGAGCATCTGTACGAATTTCGAGTTTTTGTGTCCTGTTTGTTCCACA13.0303030303030303No Hit
CAGCTTCGCGAAACCACCTGACATCAGTGAGCTTACTCGCCTGGGATTTC13.0303030303030303No Hit
CTCTTCAGCAGAATGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAG13.0303030303030303No Hit
GCGGGAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAAACGAAACTAA13.0303030303030303No Hit
ACAAAGAACAAAATTGAGAAAATAAGGCCACTTATAATAGATGGCACAGC13.0303030303030303No Hit
AGACACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATG13.0303030303030303No Hit
ATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCAA13.0303030303030303No Hit
CATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAAC13.0303030303030303No Hit
GTCAATGGACTTTATGTCTTAAATAAAATTGTTTTTCTCCTTTATATAAT13.0303030303030303No Hit
GACCGTCAGCCCCTTCAGCACGGGTTGGTCGGGGCGGTGGGGATACTCAA13.0303030303030303No Hit
GGAGACCGGTTGGAATTTCTAGCCTGGTGGAGGCCATGGTGTCTAGGGCC13.0303030303030303No Hit
GATGTCTGTATCAGGAACGCATAGACTGATAAAATTGACTCACTCCTGGC13.0303030303030303No Hit
GAGAGATCTAATGTAGCAGTCCCGCACTCGCGAGATACTCAATAAGACCA13.0303030303030303No Hit
CTACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTG13.0303030303030303No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)