FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGGATTCATTTCGTCAGTCCGAAAGAGGCGAAGAGACATTTGAA11.2658227848101267No Hit
ATGCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCA11.2658227848101267No Hit
CTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAG11.2658227848101267No Hit
AGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGCAAGGCCGTATT11.2658227848101267No Hit
GACTATGTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATTTGATTG11.2658227848101267No Hit
GTATTTTTCCCATTTGTGTGGCTCCAGTCTCGGGTCAGTGAGTGATAACT11.2658227848101267No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC11.2658227848101267No Hit
CTCATAGCCGTTGCTCAGTACGGATCAGCGGCCTCACCCTTAAGGGCCAA11.2658227848101267No Hit
CTTATAAAGTTCACCACATCAGTATCATTTCTCAAATGAGACCTTCCTTT11.2658227848101267No Hit
CTGTTATACCGTTGGTTGTAGAAGCCAAATCCCTTTTTTCATCAACTGAT11.2658227848101267No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG11.2658227848101267No Hit
TGGCTGTTTTGGAGTAATTTGAATGGGTCTATCCCGACCAGTGAGTACCC11.2658227848101267No Hit
GACTAGGGAAAGGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACA11.2658227848101267No Hit
GTACGGTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACC11.2658227848101267No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.2658227848101267No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGT11.2658227848101267No Hit
GGTCCGTGCCGGGACACTGCAGGCTGACCTGCAGGGCCTGGGGAGACGGG11.2658227848101267No Hit
TCTAGGCACCCCTAGAGTCTTTTTAAGTAGAATAAAATACAAAAGATCAA11.2658227848101267No Hit
CTTTCCCTGGGAAATCCAATATAAACTGCGTATCTGCTGACACCACCTAA11.2658227848101267No Hit
ATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAG11.2658227848101267No Hit
CTGCTGAAGAGACTGAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCA11.2658227848101267No Hit
GAATTNAATCCATCGACAATGTGATGGGAATGATCGGAATACTGCCCGAC11.2658227848101267No Hit
GTTAGGCTCTTTCCAGCCAAAGAATGTCTTCATGCATATGATTGCATCAT11.2658227848101267No Hit
GCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACGGACCAATTCTC11.2658227848101267No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT11.2658227848101267No Hit
GTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAA11.2658227848101267No Hit
AGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGC11.2658227848101267No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG11.2658227848101267No Hit
CATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACA11.2658227848101267No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACCCTAATTGATGGCCATC11.2658227848101267No Hit
GGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATCATAAG11.2658227848101267No Hit
GGTGTTCACCTGGCTGGTAGCCTGAGGGCAAGGATCTGAGGGCAGAGCTG11.2658227848101267No Hit
GCACTCCGCCTGGCCCGAAGGCTGCCCCCACCTTCCTGGGCTCAGGGGAA11.2658227848101267No Hit
GCTCCACTTTGTCATCTGAGAAGGCTTTTCTTGCTCTCGTGTACTACAGG11.2658227848101267No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11.2658227848101267No Hit
CCCTCTGGCCTCCTCGAGGCTTGGGGTCAGGGAGCGGGACTCCTGTCGCA11.2658227848101267No Hit
GTCCAGAACCTCCCCCCAGTTCCCCAAGGCCGGCCCACAGCCACATCCCC11.2658227848101267No Hit
CCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATCATTCTTATAA11.2658227848101267No Hit
ACACTAAGTGGAATGAAGATCAAAATCCTCGAATGTTCCTGGCGATGGTT11.2658227848101267No Hit
AAATAATACTGATTTAAGAAATTGAAGATGACACAAAGAAATGGAAAGCC11.2658227848101267No Hit
CCAAACTTTGATTATCCCTGCTAGAACCATAGTAAGAAGACCAGCAGTCT11.2658227848101267No Hit
GCTCTGGGGACAGCTGGGCCTCAAATAGCTTACTGGGCCAAGATGTCCGA11.2658227848101267No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA11.2658227848101267No Hit
ATTAGTATCAGATGGAGGACCAAACTAATACAATATACGGAATCTTCACA11.2658227848101267No Hit
GAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCT11.2658227848101267No Hit
CATTGAAGGGGGTTAGACAGGTATGGTAGCTGGCTCGTACGGTTATCAAA11.2658227848101267No Hit
ACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGG11.2658227848101267No Hit
GAACAGGCAGCGGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGT11.2658227848101267No Hit
GGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGGCAAGCTTGCGCATAGTT11.2658227848101267No Hit
GGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATTGCT11.2658227848101267No Hit
TCATTTGGGAAAACAACTTCCATAATTACATCCTGTGCCTCCTTGGCACT11.2658227848101267No Hit
GTATTTATTTAAACCTTGCAATGTGTCACGCTAGTCCTGGGGGCTGTGGA11.2658227848101267No Hit
TCTGTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGAC11.2658227848101267No Hit
GGCCCCGTCTGGCCTCCTGCTTAGGCAGAGACAAGCATAAACGCATGAAC11.2658227848101267No Hit
ACCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGC11.2658227848101267No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG11.2658227848101267No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT11.2658227848101267No Hit
GCCACAGTCCTCTCCCTCTGGCCTACGCGAGGCGTGGGTTCAGGTATCGG11.2658227848101267No Hit
GACCCTACAAAAAGTATTGAGTACCCCTGCACTCCGACACGTCTCCACTA11.2658227848101267No Hit
GGATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGCACC11.2658227848101267No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC11.2658227848101267No Hit
TGCAGGGGTCTTAACACCTCCAGCAAGCCCCACTGGGAGACGGGACTCGA11.2658227848101267No Hit
GTCCGGGATCATGACCTGAGCCGAAGGCAGCTACTCAACCAACTGAGCCA11.2658227848101267No Hit
CTGTTAGGCCATTCGATCTAAAGACTTCTATGGTGTTGGCCAATGCAGTT11.2658227848101267No Hit
CATTTGCACATAACAATTCCCAACAAAAACCTGCTAACACCCAGGAGGGA11.2658227848101267No Hit
TTTAAAAGTTCTCTTATACCTTGTGTTTAGCAAGGAGACGTTATAGTACA11.2658227848101267No Hit
GATCAAGCCCCACATGGGGGCTCTCTGCTCTGTAGAAGTCTCCTTCTCCC11.2658227848101267No Hit
GCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAG11.2658227848101267No Hit
GGTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATG11.2658227848101267No Hit
CTTTGCTGCTGCCCCACCAGAACAGAGTAGGATGCAATTTTCCTCATTGA11.2658227848101267No Hit
GGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATATAATAAGAGCACT11.2658227848101267No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC11.2658227848101267No Hit
TGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTT11.2658227848101267No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG11.2658227848101267No Hit
TCTTTATTCAGTAGGAGCTACTGAGTGGCTGACAGGAAGGAGAGCCCTGG11.2658227848101267No Hit
GCCTCGGGGAGCTGGGGCGGCGGGAGCCTGTATGGTGCCTGGTGTGGGAT11.2658227848101267No Hit
ATTTTAAGCACACTTCTGGAATGTGAAACGTGCGGATATTGACTAATTTT11.2658227848101267No Hit
GATGCAAAAATTCTCAACAAAATACTAGTAAACCAAAGCTAAACATACAT11.2658227848101267No Hit
ATCATAAGTCTGGCGACCTTGAGTTAGTTTATCTACCCTTGTTTGTTGAA11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAAAA50.0145.06
GAAGAAA50.0145.03
AGAAAAA50.0145.05
GGAAGAA50.0145.02
AAGAAAA50.0145.04
AAAACAA50.0145.09
GGGAAGA50.0145.01
AAAAACA50.0145.08
AAAAAAC50.0145.07
TTTGTTG50.0145.0145
Produced by FastQC (version 0.11.5)