FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGATGGAGGACCAAACTTATACAATATACGGAATCTTCACATTCCTG12.7777777777777777No Hit
CAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCTTTTTGGAAATGCCTC12.7777777777777777No Hit
GGGCAAGAATACTGACATAAGAGTCCCAGCTGGCAATAACAAAAGAGAAG12.7777777777777777No Hit
GGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCACAAGATCCC12.7777777777777777No Hit
GCATGGAAATGAGGCGCTGCCTTCATCAGTATCTTCAGCAGATAGAGAGC12.7777777777777777No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTGTGGAGGTTGCCCGTT12.7777777777777777No Hit
TTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAGA12.7777777777777777No Hit
ACTTAAACAGTATGACAGTGATGAGCCAGAGCCGAGATCTCTAGCAAGCT12.7777777777777777No Hit
ATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATAGT12.7777777777777777No Hit
AGGGAGTATTGACCGCTTTTTAAGGGTGAGAGAGCAAAGAGGGAACGTAC12.7777777777777777No Hit
CCTATATGAACTGATAGGGAAAAATTTCTAGAATAGATTGAAGAACTTAT12.7777777777777777No Hit
GCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAAT12.7777777777777777No Hit
CCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTC12.7777777777777777No Hit
ACATGCTAGAAATAGAATTGTTCCGTAAAAAAAGCTTACTCCAAGATGAC12.7777777777777777No Hit
TTCCTCGCCCAAAGATACTAGGCCCTCTGGGCGAGCGATTGGACCAGGCG12.7777777777777777No Hit
CATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTATG12.7777777777777777No Hit
AGAATTGCCTGTTACCAGTATCCTCCACCCTGATCCTCTCACATTCACAG12.7777777777777777No Hit
GTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAACACTTCAAT12.7777777777777777No Hit
AGAAAGAAGAGATCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGGAC12.7777777777777777No Hit
ATGGTGGAACAGATCTTCATGATCTCAGAGACCTCTTCTTTCATGAACCG12.7777777777777777No Hit
GGCCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGC12.7777777777777777No Hit
TCATTAATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAA12.7777777777777777No Hit
CTCTGCATCTTTGTTAATCGTATTTAATTTCATTGATCTTATTAGATAGC12.7777777777777777No Hit
AGAAGGCACAGCATCACTGCGTCCTGGTATGATGATGGGCATGTTCAACA12.7777777777777777No Hit
GTAATGAACTGTACTTGTTGTTTGGCCATTCCTATTCCACAACTTTACGG12.7777777777777777No Hit
TTACAGGAACTATGCGCAAGCTTGCAGCCCAAAGTCTCCCACCGAACTTC12.7777777777777777No Hit
TCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGA12.7777777777777777No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA12.7777777777777777No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA12.7777777777777777No Hit
CTATAAGTTAGGTCCTCCATCTGATACTAATAGCCCTAAATTTCATTGGC12.7777777777777777No Hit
AACCCTGGCCATGCAGATCTCAGTGCCAAGGATTCACAGGATGTGATTAT12.7777777777777777No Hit
CCGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCA12.7777777777777777No Hit
TCTTTCAGACCAGCACTGGAGCTAGGATGAGTCCGAATAGTTCTCATTGC12.7777777777777777No Hit
ATTTGAGAGCATCCATCAGCAGGAACTTTGACCGCGGATGGCCAAGAGGC12.7777777777777777No Hit
ACGCGGATCGGGCCTGCGGCTGCCTTTTGTTGAGTGGTATTTCCTCCACT12.7777777777777777No Hit
CCCTTATAGCATATACGGAATCTTGTCGTTCGTGAAGTCTGCTTAAAATG12.7777777777777777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)