FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339989
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA14470.10798596107878498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2700.059.0840341
CAATTCG3450.044.1296429
TATACGT1803.1977834E-936.254764
CGAAAGC4600.034.6784673
TATTACA10250.034.6630862
GTATTAC8200.034.4875221
CAGCTCG6700.033.544179
GCGAAAG4950.032.226452
AGTAGAA13550.032.1087341
GTAGAAA15000.028.5214731
CGCCAAT2553.0213414E-928.4351064
TGTATAC3403.6379788E-1227.7242262
ATAGACC7700.027.3087793
TAGAAAC16300.027.1354622
GGTCGTT2559.094947E-1225.5877747
CCTTATA5450.025.2785472
AGGTCGT2601.6189006E-925.099456
TATATAC19400.025.0419463
GTATTAT7900.024.782661
GTATTAG2351.3290501E-624.685011
Produced by FastQC (version 0.11.5)