FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTAGAGAGGCAGAGCAGGGAGCCCAAAGGGTAGTTTATATGAGAAAG30.22271714922048996No Hit
GTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGAAAATT30.22271714922048996No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA20.14847809948032664No Hit
GCATCATAGGAAATGTGTGATACATTTTAGAATGGATTCAAGAGCTGTTA20.14847809948032664No Hit
CACAACATTGTATGTAGACAGATGAAAGTCTTTTGAAAACTGAAAATCAC20.14847809948032664No Hit
CCCCTAGAGTGTTTTGAAGTAGAATAAAATACAAAGGATCAAAAGAACAC20.14847809948032664No Hit
ATCCAAAAGACTTAGCAATCCCAAAGAAGGATTCCCTTTTAAGTAGTCAC20.14847809948032664No Hit
CATTTACCCTCAAGTTCTTTTTTATGATAGTCAGGGAAAGTATCCCATTT20.14847809948032664No Hit
GCAGTAGGCAGGGGTTTTAGAGAGGCAGAGCAGGGAGCCCAAAGGGTAGT20.14847809948032664No Hit
GGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAAGC20.14847809948032664No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA20.14847809948032664No Hit
TTCCACGACCTGGGGCTCCAACACACAACACTAAGGGCAAGAGTCACACG20.14847809948032664No Hit
GCAAAGAAGGGAGGAGGATAGGAAAAGACTTTAACCAGGAAGGAGCTGGA20.14847809948032664No Hit
CTCCTGAGGGCAGACTGTCTTGCTTGTACTTGCGGAGGACTCCCGTGGCC20.14847809948032664No Hit
CCAGGCACGTTTCCCAGCCCTGGGGCTGCCACAGGCCCACACAGGGTGGG20.14847809948032664No Hit
TAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGA20.14847809948032664No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)