FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_A02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_A02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195742
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT25550.21367485628170624No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA23460.19619616940778195No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA22090.1847388483468842No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC13480.11273334883277497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3600.096.688721
GCGAAAG5400.064.456452
CGAAAGC5800.060.011183
TATACAG4350.041.674435
GTACGTA1604.591857E-836.2491769
ACCTATC11300.034.645238
GTAAGCG1551.4631933E-632.7480353
GCGTAGC1402.7475493E-531.070727
TTACATC10200.030.569424
GTATTAC13150.030.3301121
TATATAC8950.029.9776523
TAATACC14550.028.905734
GTAATAC14600.028.3100683
CGTAAAA1306.476638E-427.889812
CCTATCA14100.027.7653269
TACATCA10800.027.5282765
TAAGCGT1606.820577E-527.1925684
TATTACA13150.027.0202432
CAACGCC1358.0807693E-426.853485145
CTAGGCT6900.026.273014
Produced by FastQC (version 0.11.5)